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Use of colloidal gold immunological chromatography in detection of aquatic, agricutural and livestock products

An immunochromatography, immunochromatography technology, applied in the field of aquatic products, agricultural and livestock products detection, agricultural and livestock products detection, colloidal gold immunochromatography in the water field, can solve the limited conditions, detection To solve the problem of single hand, achieve the effect of low cost, high sensitivity and strong specificity

Inactive Publication Date: 2003-04-09
张少恩
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Problems solved by technology

[0005] The present invention aims at the problems such as single detection of aquatic products, agricultural products and livestock products and limited conditions, etc., and introduces colloidal gold immunochromatography technology into the detection field of aquatic products, agricultural products and livestock products

Method used

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  • Use of colloidal gold immunological chromatography in detection of aquatic, agricutural and livestock products
  • Use of colloidal gold immunological chromatography in detection of aquatic, agricutural and livestock products

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Embodiment Construction

[0015] The present invention will be further described below in conjunction with accompanying drawing and embodiment:

[0016] see figure 1 , application of the present invention mainly comprises the following steps: 1. raw material preparation

[0017] 1) Preparation of monoclonal antibody: The hybridoma cell line was routinely cultured and passaged in vitro, injected into the peritoneal cavity of BALB / C mice with the small molecule carrier to be tested, collected ascites, and purified monoclonal antibody by affinity chromatography.

[0018] 2) Preparation of polyclonal antibody: immunize goats or rabbits with the small molecule-carrier to be detected multiple times, extract and purify the antiserum to obtain goat anti-mouse IgG or rabbit anti-mouse IgG.

[0019] 3) Preparation of colloidal gold: reducing chloroauric acid into colloidal gold particles with a reducing agent such as trisodium citrate.

[0020] 4) Colloidal gold labeling of monoclonal antibodies: Mix colloidal...

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Abstract

A colloidal gold immunity chromatograph to be used for the inspection of aquatic product, agricultural product and livestock product belongs to the technical field of biological medicine. The presentinvention with high sensitiivty and high specificity and be widely used in the departments of inspection and epidemic prevention to overcome the problems such as short of inspecting means and limitation by the conditions existed in the product inspection for the quatic, and livestock agricultural prdoucts.

Description

1. Technical field [0001] The invention relates to detection technology for aquatic products, agricultural products and animal products, in particular to the application of colloidal gold immunochromatography in the detection field of water, agricultural and animal products, and belongs to the technical field of biomedicine. 2. Background technology [0002] In the prior art, the detection of aquatic products, agricultural products and animal products usually adopts chromatographic technology. Because of its high sensitivity, high separation efficiency and high selectivity, it has almost become a necessary analysis method in modern analytical laboratories in various industries. [0003] However, chromatographic technology has the following defects: 1) the sample pretreatment procedure is cumbersome and demanding; 2) there are strict requirements for water and various solvents used in operation; 3) the operation process is complicated and takes a long time; 4) it requires Ope...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/558
Inventor 张少恩
Owner 张少恩
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