Biological gold extraction method of difficult separation concentrate and special equipment
A concentrate and refractory technology, applied in the field of metallurgy, can solve the problems of difficult safe operation, environmental pollution, and high cost, and achieve the effects of no environmental pollution, saving water resources, and improving recovery rate
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Embodiment 1
[0023] Example 1, see figure 1 , 2 , 3, first carry out the activation of bacterial inoculum and expand culture, the bacterium that adopts is a kind of mesophilic mixed bacteria, mainly comprises Thiobacillus ferrooxidans 26%, Thiobacillus thiooxidans 17%, Helicobacter ferrooxidans 57%. The mixed bacteria is collected from the mine pit, the working temperature is 40 degrees, the pH value is 0.8-1.2, and the number of activated bacteria is 10 8 -10 9 per milliliter culture solution, the oxidation rate of sulfur in gold concentrate is 85%.
[0024] Adopt the following step-by-step scale-up culture method:
[0025] Primary culture: Shake flask culture in the laboratory;
[0026] Secondary cultivation: 3 cubic meters of stainless steel tank cultivation;
[0027] Tertiary cultivation: 80 cubic meters of stainless steel tank cultivation; among them, the second and third cultivations need to meet the following conditions:
[0028] a. The particle size of gold concentrate should...
Embodiment 2
[0041] Embodiment 2, different from Example 1, is that in the activation and expansion culture process of the bacterial inoculum, the bacteria used are a kind of mesophilic mixed bacteria, which mainly include Thiobacillus ferrooxidans 34%, Thiobacillus thiooxidans 10% %, Spirulina ferrooxidans 58%.
Embodiment 3
[0042] Embodiment 3, different from Example 1, is that in the activation and expansion culture process of the bacterial inoculum, the bacteria used are a kind of mesophilic mixed bacteria, which mainly include Thiobacillus ferrooxidans 31%, Thiobacillus thiooxidans 15% %, Spirulina ferrooxidans 54%.
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