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Fluorescence detecting system for quantitative gene magnification instrument

A technology for fluorescence detection and gene amplification, applied in the field of fluorescence detection systems, can solve the problems of complex metals, large scattering and attenuation of light intensity, and high cost, and achieve the effects of reducing manufacturing costs and improving detection sensitivity.

Inactive Publication Date: 2003-06-11
SUZHOU MOLARRAY CO LTD +1
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Problems solved by technology

At the same time, due to the complexity of metals that receive fluorescence from the upper part of the base, the fluorescence will scatter and attenuate greatly when passing through the liquid to the space and from the air to the reaction tube, resulting in low detection sensitivity and high cost

Method used

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  • Fluorescence detecting system for quantitative gene magnification instrument
  • Fluorescence detecting system for quantitative gene magnification instrument
  • Fluorescence detecting system for quantitative gene magnification instrument

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Embodiment Construction

[0010] Quantitative gene amplification instruments are generally composed of a fluorescence detection system, a data processing system and a display system. The fluorescence detection systems of the existing quantitative gene amplification instruments are such as figure 1 1-1 is the excitation light, 1-2 is the fluorescence emission, 1-3 is the sample, 1-4 is the sample base, 1-5 is the cap scattered light, 1-6 is the cap, 1- 7 is the liquid surface scattered light, 1-8 is the sample liquid level. When the excitation light is reflected by the sample and reaches the interface 1-8 between the sample liquid and the air, part of the light scattering 1-7 occurs, and then when the reflected light reaches the cap 1-6, light scattering 1-5 occurs again, thus The intensity of light is greatly weakened, which affects the sensitivity of detection.

[0011] The quantitative gene amplification instrument of the present invention is also composed of a fluorescence detection system, a data ...

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Abstract

The fluoroscopic examination system of quantitative gene augmentor is composed of the light source with the color filter, the two-way mirror positioned in front of the light source inclined 45 degrees, the 96 optical fiber bundle coaxial with the light source and the corresponding sample cells. Each optical fiber bundle is leaded in the pinhole in the bottom of the base in each sample cell. The excitation and the reception of fluorescence are realized directly on the wall of the reaction tube. Thus, the phenomena of the fluorescence scattering and the light intensity attenuation are eliminated. The invention raises the detection sensitivity and reduces the manufacturing cost so as to possess the application value.

Description

technical field [0001] The invention relates to biological and medical detection instruments, in particular to a fluorescence detection system of a fluorescence quantitative gene amplification instrument. Background technique [0002] The fluorescence detection system of the existing quantitative gene amplification instrument is to irradiate the laser light into the sample tube from the upper part of the detection sample base, and then collect the instantaneously excited fluorescence in the sample tube from the upper part of the base to realize the detection. In this way, the quality of the cap of the sample reaction tube is particularly high, otherwise the optical path will be confused. At the same time, due to the complicated metal that receives the fluorescence from the upper part of the base, the fluorescence is scattered and the light intensity is greatly attenuated when passing through the liquid to the space and from the air to the reaction tube, resulting in low dete...

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 吴元民张凯罗氏立格聂晓峰
Owner SUZHOU MOLARRAY CO LTD