Immunity colloidal gold reagent for detecting IgM antibody of herpes simplex virus II type and its preparing method

Abstract

The sample and the binding pad, which is the conjugate coated with the anti U chain monoclonal antibody and colloidal gold, are adhered to the one end with PVC back covered in sequence. The cellulosenitrate film coated with the specificity surface film antigen of herpes simple virus II type and the anti rat IgG of sheep (or rabbit) is adhered to the middle of the reagent. The water uptake pad isadhered to another end of the reagent. The reagent features high specificity and sensitivity, simple and fast. The reagent is applicable to the detection in site within 30 minutes for the full testing procedure. Operation personal can complete the operation by reading the menu and without need of professional training.

Description

Technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] The existing methods used to detect the herpes simplex virus ‖ type antibody IgM mainly include enzyme-linked immunosorbent assay, radioimmunoassay, immunogold percolation method, western blotting test (WBA), etc. The disadvantages of the enzyme-linked immunosorbent (EIA) method are: it requires special equipment such as microplate reader to cooperate with it; the detection operator needs to be professionally trained; the operation process is relatively complicated and the detection takes a long time; the cost of the detection It is higher and cannot realize single test. The disadvantages of the immunocolloidal gold percolation method are: the operation process is relatively complicated and one-step operation cannot be realized; the reagents need to be stored at a low...

AI Technical Summary

Problems solved by technology

The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection High, unable to achieve single-person detection

The disadvantages of immunocolloidal gold diafiltration are: the operation process is relatively complicated, and one-step operation cannot be realized; reagents need to be stored at low temperature; the cost of detection is high; the time required for detection is longer than that of colloidal gold immunochromatography

The disadvantages of radioimmunoassay are: operators need to undergo professional training; the operation process is complex and demanding; reagents need to be stored at low temperature; because the test involves radioactive isotopes, the test is not very safe, so it is rarely used; the test cost is high; takes a long time

The defect of western blotting assay (WBA) is: the operator needs to be through professional training; Limited to some professional laboratories; high cost of testing; long time required for testing