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Potato 1-amino-cyclopropane-1-carboxylic-synthesized zyme gene chip and primer

A technology of aminocyclopropane and gene fragments, applied in the field of nucleic acids

Inactive Publication Date: 2003-08-27
MOLECULARTAQ LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether food (including genetically modified food) contains potato ingredients, there is no simple and feasible determination method at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] Synthesis of ACC primers

[0019] Primers were prepared according to conventional methods well known in the art. For example, the synthesis is carried out on an automatic DNA synthesizer (such as ABI3949 automatic DNA synthesizer from PE Company, USA) according to the manufacturer's instructions. The forward primer 5'-gctgaggagtgctgggatt-3'(SEQ ID NO:2) of potato ACC synthase gene and the reverse primer 5'-ccttgtttccatgaaatggtg-3'(SEQ ID NO:3) of potato ACC synthase gene were synthesized respectively.

[0020] Amplification of 265bp DNA fragment of ACC gene

[0021] Potato DNA was extracted with the Qiagen DNeasy Plant Mini kit according to the manufacturer's instructions and dissolved in 100 microliters of double distilled water. Take 5 μl of DNA and add PCR master mix (1x PCR buffer, 200 μmol dNTPs, 200 μmol potato ACC forward and reverse primers and 0.25 units Taq DNA polymerase). The PCR buffer is: 10mM Tris-HCl (pH8.6), 50mM KCl, 1.5mM MgCl 2 and 0.1% Triton X-...

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Abstract

The invention provides a DNA segment obtained by peculiar PCR increase in potato and PCR guide matter. The DNA segment and guide matter can peculiarly identify DNA component of potato.

Description

technical field [0001] The present invention relates to nucleic acids, in particular, the present invention relates to potato-specific nucleic acids. Background technique [0002] Polymerase chain reaction (PCR) is widely used in various DNA-based assays. However, in order to ensure the success of PCR analysis, the key lies in the quality and quantity of the template DNA and the degree of specificity of the primers used. [0003] DNA from different sources, such as DNA from various processed foods, is often contaminated by proteins, fats, polysaccharides, polyphenols, and other substances that may interact with DNA polymerases and nucleic acids to affect PCR analysis, and even in some cases It can also lead to false negative results. [0004] Potatoes are widely used in food, such as French fries, potato chips, mashed potatoes, bread, chocolate, frozen food, etc. It is estimated that processed foods containing potatoes make up 5-10 percent of all foods. Whether food (inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/00C07H21/04C12N15/52C12P19/34C12Q1/68
Inventor 王骏黄蕙薇
Owner MOLECULARTAQ LTD