Method for preparing grifola fungus seeds

A production method and technology for strains, applied in the fields of botanical equipment and methods, fungi, horticulture, etc., can solve the problems of survival, low success rate, production limitation of Polyporus chinensis, low product yield, etc., and achieve fast sclerotia formation. , significant social and economic benefits, short production cycle

Inactive Publication Date: 2003-10-08
杨海晏
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Problems solved by technology

[0002] For a long time, the production of Polyporus sinensis has been cultivated and produced by using wild Polyporus tuber as a seed. This production method has restricted the commercial production, development and utilization of Polyporus sinensis. It is mainly manifested in the following aspects: 1. Low survival and success rate Generally, the survival and success rate is only about 20%. The survival and success rate are mainly determined by factors such as the freshness of the tubers, the age of the tubers, the season of excavation and cultivation, altitude, sunshine, temperature, soil humidity, and degree of shading. 1. The success rate is greatly affected

Method used

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Examples

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Embodiment

[0024] Example: Firstly, at a temperature of 15°C to 25°C, separate and cultivate tubers in conventional PDA medium, purify and rejuvenate in enriched PDA medium, form sclerotia within 25 days, and then use secondary and tertiary sawdust Wheat bran is used as a medium (conventional medium), and sclerotia can grow in about 30 days in the second-level wood chip wheat bran medium, and sclerotia can grow in about 40-60 days in the third-level wood chip wheat bran medium. The largest single sclerotia formed in the high-grade sawdust wheat bran medium reached 30 grams.

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Abstract

A process for preparing the seed of umbrellate pore-fungus includes separating stem tuber, culturing in conventional PDA culture medium at 15-25 deg.C, purifying and rejuvenation in PDA enriched culture medium for 25 days to form selerotia, and culturing the selerotia in the saw dust-wheat bran culture medium for 30-60 days. Its advantages are high yield, short period, high survival rate.

Description

1. Technical field: [0001] The invention relates to a method for cultivating and preparing strains, in particular to a method for preparing strains of Polyporus. 2. Background technology: [0002] For a long time, the production of Polyporus sinensis has been cultivated and produced by using wild Polyporus tuber as a seed. This production method has restricted the commercial production, development and utilization of Polyporus sinensis. It is mainly manifested in the following aspects: 1. Low survival and success rate Generally, the survival and success rate is only about 20%. The survival and success rate are mainly determined by factors such as the freshness of the tubers, the age of the tubers, the season of excavation and cultivation, altitude, sunshine, temperature, soil humidity, and degree of shading. 1. The success rate is greatly affected by external factors; 2. The output of the product is low, and the conversion rate of every 100 jin of trunks is only about 20%; 3...

Claims

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Application Information

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IPC IPC(8): A01G1/04C12N1/14
Inventor 杨海晏
Owner 杨海晏
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