Method for preparing grifola fungus seeds

Abstract

A process for preparing the seed of umbrellate pore-fungus includes separating stem tuber, culturing in conventional PDA culture medium at 15-25 deg.C, purifying and rejuvenation in PDA enriched culture medium for 25 days to form selerotia, and culturing the selerotia in the saw dust-wheat bran culture medium for 30-60 days. Its advantages are high yield, short period, high survival rate.

Description

1. Technical field: [0001] The invention relates to a method for cultivating and preparing strains, in particular to a method for preparing strains of Polyporus. 2. Background technology: [0002] For a long time, the production of Polyporus sinensis has been cultivated and produced by using wild Polyporus tuber as a seed. This production method has restricted the commercial production, development and utilization of Polyporus sinensis. It is mainly manifested in the following aspects: 1. Low survival and success rate Generally, the survival and success rate is only about 20%. The survival and success rate are mainly determined by factors such as the freshness of the tubers, the age of the tubers, the season of excavation and cultivation, altitude, sunshine, temperature, soil humidity, and degree of shading. 1. The success rate is greatly affected by external factors; 2. The output of the product is low, and the conversion rate of every 100 jin of trunks is only about 20%; 3...

AI Technical Summary

Problems solved by technology

[0002] For a long time, the production of Polyporus sinensis has been cultivated and produced by using wild Polyporus tuber as a seed. This production method has restricted the commercial production, development and utilization of Polyporus sinensis. It is mainly manifested in the following aspects: 1. Low survival and success rate Generally, the survival and success rate is only about 20%. The survival and success rate are mainly determined by factors such as the freshness of the tubers, the age of the tubers, the season of excavation and cultivation, altitude, sunshine, temperature, soil humidity, and degree of shading. 1. The success rate is greatly affected by external factors; 2. The output of the product is low, and the conversion rate of every 100 jin of trunks is only about 20%; 3. The cost is high: use 5 jin of tubers and a bottle of fungus respectively to plant 100 jin of trees , also excavated in three years, the output of using tubers as seeds is only about 60% of that of strains; 4, the production cycle is long, and generally more than three years can be excavated; 5, the provenance is very limited, only a small amount Wild and clonal tubers

The above-mentioned unfavorable factors lead to many restrictions on the production of Polyporus umbilicalis, which makes the supply of goods on the market very scarce.