Dermoid trichocyst yeast fermentation process of producing pyruvic acid
A technology of Trichosporium dermoides and pyruvic acid, applied in the biological field, can solve the problem of high production cost
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Embodiment 1
[0007] Trichosporium dermatitis 9070 (CGMCC0754) was inoculated in 30ml fermentation medium 1 contained in a 250ml shake flask, and the medium components were glucose 20%, peptone 5%, KH 2 PO 4 0.5%, MgSO 4 0.1%, CaCO 3 5%. After fermenting at 30° C. for 3 days, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 40.1 g / L of pyruvate.
Embodiment 2
[0009] Trichosporium dermatosa 9070 (CGMCC0754) was inoculated in 25ml fermentation medium 2 packed in a 250ml shake flask, and the medium components were glucose 5%, beef extract 5%, KH 2 PO 4 0.1%, MgSO 4 0.02%, CaCO 3 1%. After fermenting at 24°C for 1 day, take 10ml of fermentation broth and inoculate 75ml of fermentation medium 3 in a 250ml shaker flask. 2 PO 4 0.15%, MgSO 4 0.07%, CaCO 3 5%. After fermenting at 28° C. for 3 days, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 42.3 g / L of pyruvate.
Embodiment 3
[0011] Trichosporium dermidis 9070 (CGMCC0754) was inoculated in 50ml fermentation medium 4 contained in a 250ml shake flask, and the medium components were molasses 8%, corn steep liquor 2%, KH 2 PO 4 0.05%, MgSO 4 0.01%, CaCO 3 1%. After fermenting at 32° C. for 1 day, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 32.8 g / L of pyruvate.
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