Dermoid trichocyst yeast fermentation process of producing pyruvic acid

A technology of Trichosporium dermoides and pyruvic acid, applied in the biological field, can solve the problem of high production cost

Inactive Publication Date: 2004-01-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the method for above-mentioned production pyruvic acid all needs to add

Method used

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Effect test

Embodiment 1

[0007] Trichosporium dermatitis 9070 (CGMCC0754) was inoculated in 30ml fermentation medium 1 contained in a 250ml shake flask, and the medium components were glucose 20%, peptone 5%, KH 2 PO 4 0.5%, MgSO 4 0.1%, CaCO 3 5%. After fermenting at 30° C. for 3 days, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 40.1 g / L of pyruvate.

Embodiment 2

[0009] Trichosporium dermatosa 9070 (CGMCC0754) was inoculated in 25ml fermentation medium 2 packed in a 250ml shake flask, and the medium components were glucose 5%, beef extract 5%, KH 2 PO 4 0.1%, MgSO 4 0.02%, CaCO 3 1%. After fermenting at 24°C for 1 day, take 10ml of fermentation broth and inoculate 75ml of fermentation medium 3 in a 250ml shaker flask. 2 PO 4 0.15%, MgSO 4 0.07%, CaCO 3 5%. After fermenting at 28° C. for 3 days, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 42.3 g / L of pyruvate.

Embodiment 3

[0011] Trichosporium dermidis 9070 (CGMCC0754) was inoculated in 50ml fermentation medium 4 contained in a 250ml shake flask, and the medium components were molasses 8%, corn steep liquor 2%, KH 2 PO 4 0.05%, MgSO 4 0.01%, CaCO 3 1%. After fermenting at 32° C. for 1 day, the bacterial cells were separated by centrifugation, and the obtained supernatant contained 32.8 g / L of pyruvate.

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Abstract

Pyruvic acid is one important intermediate metabolite of glycometabolism of living body, has certain health functions and is the material for synthesizing L-tryptophane, L-tyrosine, L-dopa and other products. The present invention is the method of producing pyruvic acid via fermentation of glycose with dermoid trichocyst yeast. Inside simple culture medium without vitamins and amino acids added, pyruvic acid over 40 g/L may be obtained. The present invention has low material cost and excellent application foreground.

Description

technical field [0001] The invention belongs to the field of biotechnology, and is a new process for producing organic acids by yeast fermentation. Specifically, the present invention relates to a method for fermentatively producing pyruvate using Trichosporium dermatosa yeast. Background technique [0002] Pyruvic acid is an important intermediate metabolite of sugar metabolism in organisms, and has health-care and physiological functions (Pyruvic acid's health function. Soft Drinks International, 1998 (5): 24.), and is the synthesis of L-tryptophan, L-tyrosine , L-dopa and other products of raw materials. Pyruvate can be obtained by microbial fermentation of glucose (Qiang Hua, Chen Yang, kazuyuki Shimizu: J. Biosci. Bioeng.. 1999, 87 (2): 206-213; A. Yokota, H. Shimizu, Y. Terasawa et al: Appl .Microbiol.Biotech..1994, 41:638-643; Mitsuaki Moriguchi: Agric.Biol.Chem..1982, 46(4):955-961; M.Moriguchi, K.Shuto, T.Hashimoto: J.Ferment .Technol..1984, 62(3):243-248; S.Taka...

Claims

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Application Information

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IPC IPC(8): C12P7/50
Inventor 江宁王钦宏沈安贺鹏卢大军
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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