Snake venom enzyme nerve growth factor for treating diabetic concurrent peripheral nervous pathological complication
A technology for peripheral neuropathy and nerve growth factor, applied in the direction of nerve growth factor, growth factor/inducer, nervous system diseases, etc., can solve the problem of subjective symptoms not being eliminated
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[0017] Embodiment preparation process is:
[0018] All operations were performed below 10°C.
[0019] ① CM-Sephadex C50 chromatography
[0020] Take 10g of cobra venom, dissolve it in 100ml of 0.01M sodium acetate buffer solution, pH5.8, after dissolving, centrifuge at 10000 rpm for 10 minutes, take the supernatant, dialyze against 5000ml of the above buffer solution, change the buffer solution 3 times, and then put the sample on The sample was equilibrated with 0.01M acetate buffer solution in a 5.5×100cm chromatographic column, eluted with a linear gradient of 0-0.7M NaCL, and the protein peak with nerve growth factor activity was collected, concentrated and freeze-dried.
[0021] ③ CM-Spharose FF chromatography
[0022] The above-mentioned concentrated sample was dissolved in 20ml of 0.01M sodium acetate buffer solution, pH5.8, and loaded on a chromatographic column 2.6×50cm equilibrated with 0.01M sodium acetate buffer solution pH5.8, linearized with 0-1M NaCl Gradient ...
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