Pterocarya stenoptera extract, its preparation and use

A kind of use and compound technology, applied in the field of maplene and its preparation, can solve the problem of no natural diphenylepoxyheptane compounds, etc., and achieve the effect of inhibiting the cell cycle

Inactive Publication Date: 2004-11-17
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Diphenylheptanoid glucosides have been reported in some literature, such as [Motohiko Morihara, e.al., Twonovel diarylheptanoid glucosides from Myrica gale var.tomentosa and absolute structure of plane-chiral galleon.Chem.Pharm.Bull., 1997, 45(5): 820-823] and literature [Gabriela IslasGonzalez, et al., A unified strategy toward the synthesis of acerogenin-type macrocycles: Totalsyntheses of acerogenina A, B, C, and L and aceroside IV.J. Org.Chem., 1999, 64, 914-924] have reported the natural and synthetic compounds of diphenylepoxyheptanes respectively, but there are no reports that this type of compounds have cell cycle inhibition and apoptosis induction activity
There is also no literature report on the isolation of natural diphenylepoxyheptane compounds from Tokyo maple poplar

Method used

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  • Pterocarya stenoptera extract, its preparation and use
  • Pterocarya stenoptera extract, its preparation and use
  • Pterocarya stenoptera extract, its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Separation and purification of compound I

[0021]

[0022] Compound I

[0023] In the formula, the Arabic numerals are the positions of the carbon atoms in the chemical structure.

[0024] Get the dry stem bark (3.5 kg) of Tokyo maple poplar and pulverize it, soak it with 10 liters of 60% ethanol at room temperature for 6 days, filter the aqueous ethanol solution (the same extraction is carried out 8 times), combine the extracts, and concentrate under reduced pressure until it does not contain Ethanol (about 2 liters), extracted 4 times with an equal volume of chloroform, combined the chloroform extracts, concentrated and dried to obtain 10.4 g of chloroform extracts.

[0025] Get the chloroform extract (10.4 grams), add 10 grams of thin-layer chromatography silica gel G after dissolving with appropriate amount of chloroform and mix the sample, use 194 grams of silica gel H dry method to go up the decompression column, use chloroform→metha...

Embodiment 2

[0058] Example 2 Tests for Inhibitory Activity and Apoptosis-Inducing Activity of Cancer Cell Proliferation and Cell Cycle

[0059] 1 Experimental samples and experimental methods

[0060] Preparation of the tested sample solution Take the pure compound I isolated and refined in the above-mentioned Example 1, accurately weigh an appropriate amount, and prepare a solution of the required concentration with methanol for testing the activity.

[0061] Cell lines and cell culture The activity test uses mammalian cancer cell lines such as mouse breast cancer tsFT210 cells, human chronic myelogenous leukemia K562 cells and human colorectal cancer HCT-15 cells. All kinds of cells were subcultured in RPMI-1640 medium containing 10% FBS at 32°C (tsFT210 cells) or 37°C (K562 cells and HCT-15 cells) in an incubator filled with 5% carbon dioxide.

[0062] Cell Proliferation Inhibitory Activity Test Method (SRB Method)

[0063] This method adopts the SRB (sulforhodamine B, Lissamine rhod...

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Abstract

The invention relates to a pterocarya stenoptera extract, its preparation and use, wherein a novel compound of diphenyl epoxy heptane is extracted from Tokyo pterocarya stenoptera. Experiment has proved that the compound can be used as cell cycle depressor, cell apoptosis inducer or antineoplastic agent.

Description

Technical field: [0001] The invention relates to Pterocarine and its preparation method and use. It specifically relates to a new compound of diphenylepoxyheptane extracted from Tokyo maple poplar, the compound and its preparation method, and this kind of substance is used as a cell cycle inhibitor, an apoptosis inducer or a tumor cell killing agent, that is, an anti-tumor use of the agent. Background technique: [0002] Diphenylheptanoid glucosides have been reported in some literature, such as [Motohiko Morihara, e.al., Twonovel diarylheptanoid glucosides from Myrica gale var.tomentosa and absolute structure of plane-chiral galleon.Chem.Pharm.Bull., 1997, 45(5): 820-823] and literature [Gabriela IslasGonzalez, et al., A unified strategy toward the synthesis of acerogenin-type macrocycles: Totalsyntheses of acerogenina A, B, C, and L and aceroside IV.J. Org.Chem., 1999, 64, 914-924] have reported the natural and synthetic compounds of diphenylepoxyheptanes respectively, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/335A61P35/00C07D313/00
Inventor 崔承彬刘红兵蔡兵顾谦群张冬云管华诗
Owner OCEAN UNIV OF CHINA
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