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Application of S-propargyl-cysteine to preparation of medicines for treating blood disease system tumors

A cysteine, blood system technology, applied in the field of propargyl cysteine ​​in the preparation of drugs for the treatment of hematological system tumors, can solve the problems that have not yet been reported on propargyl cysteine ​​against hematological system tumors

Active Publication Date: 2019-03-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have reported that SPRC protects cardiomyocytes from hypoxic damage and its role in the treatment of digestive system tumors and breast cancer, but so far, there has been no report on the anti-hematological system tumors of propargyl cysteine ​​(SPRC)

Method used

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  • Application of S-propargyl-cysteine to preparation of medicines for treating blood disease system tumors
  • Application of S-propargyl-cysteine to preparation of medicines for treating blood disease system tumors
  • Application of S-propargyl-cysteine to preparation of medicines for treating blood disease system tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Cell Lines and Culture Mode

[0021] Human T lymphocytes (Jurkat) and lymphoma cells (Raji) cells (purchased from the cell bank of Shanghai Institute of Biological Sciences, China). Culture conditions: respectively culture Jurkat and Raji cells in RPMI 1640 containing 10% fetal bovine serum and 1-2% penicillin and streptomycin (100U / ml), and place the cells in a 5% CO2 incubator at 37°C.

Embodiment 2

[0022] Example 2 SPRC reduces cell viability experiment

[0023] 5*10-3 Jurkat and Raji cells were seeded in a 96-well plate with a volume of 200ul per well. Add SPRC to final concentrations of 0.16mg / ml, 0.8mg / ml, 1.6mg / ml and 3.2mg / ml respectively. After 48 hours of treatment, the cell viability was detected by the CCK-8 method, and the results were as follows: figure 1 As shown, the cell viability of 0.16mg / ml, 0.8mg / ml, 1.6mg / ml and 3.2mg / ml SPRC treatment groups were significantly lower than that of the normal control group, and SPRC can inhibit the growth of T lymphocytes and lymphoma cells. vitality.

Embodiment 3

[0024] Example 3 SPRC promotes cell apoptosis experiment

[0025] The Jurkat and Raji cells were seeded in culture dishes and treated with 0.8 and 1.6 mg / ml SPRC for 48 hours. Annexin I and PI stained cells were detected by flow cytometry and the results were as follows: figure 2 As shown, the results of flow cytometry showed that 0.8mg / ml SPRC can induce cell apoptosis, and 1.6mg / ml SPRC can significantly induce cell apoptosis.

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Abstract

The invention belongs to the field of pharmaceutical, and relates to an application of SPRC (S-propargyl-cysteine) to preparation of medicines for treating blood disease system tumors. According to the application, culture experiments of in-vitro T lymphocyte and lymphoma cells are implemented to detect influence of SPRC processing on cell activity of cancer cells. Results show that SPRC can reduce cell survival rate, facilitate apoptosis and inhibit S-phase arrest of a cell cycle, apoptosis is improved, and the cell activity of a protein caspase3 is facilitated. Experimental results show thatthe S-propargyl-cysteine has an inhibition effects on the T lymphocyte and the lymphoma cells, and the SPRC can be prepared into treatment medicines for treating blood disease system tumors.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to a new drug application of propargyl cysteine ​​(SPRC, S-propargyl-cysteine), in particular to the use of propargyl cysteine ​​(SPRC) in the preparation of drugs for treating hematological system tumors use. Background technique [0002] The prior art discloses that allyl cysteine ​​(SAC, S-allycysteine) is a natural organic sulfur compound in garlic, and is the main active ingredient in mature garlic extract (Aged garlic extract), which is obtained by S-alk (en ) Degradation of ylcysteine ​​sulfoxides (ACSs). It has been reported in the literature that this compound has antitumor, antibacterial and antifungal properties. [0003] According to research reports, propargyl cysteine ​​(SPRC, S-propargyl-cysteine) is similar in structure to SAC (S-allycysteine) and has the same cysteine ​​structure. Studies have reported that SPRC protects cardiomyocytes from hypoxic damage and its role in th...

Claims

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Application Information

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IPC IPC(8): A61K31/198A61P35/02
CPCA61K31/198
Inventor 朱依谆茅以诚马蓓蕾
Owner FUDAN UNIV
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