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Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture

A technology of perfusion culture and interception device, which is applied in tissue cell/virus culture devices, tissue culture, biochemical instruments, etc., which can solve the problem of destroying the steady state of the sedimentation process, increasing the processing difficulty and material cost of the settler, and long system stabilization time and other problems, to avoid clogging and failure problems, alleviate cell damage, and shorten the residence time

Inactive Publication Date: 2004-11-17
上海伯瑞生物技术发展有限公司 +1
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Problems solved by technology

When the width-to-height ratio of the settler section is too small (5), a large number of cells in the suspension are easy to deposit on the bottom of the settler, which is not conducive to the settlement of the cells. Recycle
[0008] The determination of the length of the settler is based on the average sedimentation rate of the cells and is designed through model calculation. For hematopoietic cells, if the length of the settler is too small, the cell retention capacity of the settler will be weakened, and some smaller cells will overflow with the supernatant; However, if the length of the settler is too large, it will not only increase the processing difficulty and material cost of the settler, but also require a longer system stabilization time during operation.

Method used

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  • Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture
  • Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture
  • Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture

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Embodiment 1

[0033] Cell isolation and culture: mononuclear cells (MNCs) were isolated from fresh umbilical cord blood (taken from China Welfare Institute Maternal and Child Health Hospital), resuspended in IMDM medium (Gibco BRL, USA) containing 20% ​​FBS, added SCF, IL -3 and IL-6 three kinds of cytokines, the concentrations were: 50ng / ml, 20ng / ml and 40ng / ml, with 1×10 6 cells / ml were inoculated into spinner bottles and placed at 37°C, 5% CO 2 , Humidity-saturated carbon dioxide incubator for future use.

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Abstract

A cell depositing interception device of hematopoietic cell continuous perfusion cultivation bioreactor system, comprises an inclined tube-form depressor with tube-form cross-section, the upper portion of which is arranged with a feed inlet, a discharge port is arranged on the bottom of the depressor corresponding to the feed inlet position, the upper surface of the other end is provided with at discharge port and an exhaust port.

Description

technical field [0001] The invention relates to a cell retaining device for a continuous perfusion culture bioreactor system for hematopoietic cells, in particular to a sedimentation separation device for retaining hematopoietic cells in the continuous perfusion culture process. Background technique [0002] Umbilical cord blood hematopoietic stem cell transplantation has a good application prospect in the treatment of hematopoietic dysfunction and the recovery of hematopoietic ability in patients with malignant tumors after radiotherapy. However, due to the limitation of umbilical cord blood sources, the insufficient number of umbilical cord blood hematopoietic stem cells has become a bottleneck in the research and clinical application of umbilical cord blood hematopoietic stem cell transplantation, so the research on large-scale expansion of umbilical cord blood hematopoietic stem cells in vitro has become very urgent. The perfusion culture process is considered to be an e...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/08
Inventor 谭文松姜华蔡海波迟占有
Owner 上海伯瑞生物技术发展有限公司
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