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Pterocarya stenoptera quinone, its preparation and use

A use and compound technology, applied in the field of maplequinone and its preparation, can solve the problems that have not been reported in the literature on the separation of natural quinone compounds

Inactive Publication Date: 2005-01-26
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Natural quinones can be isolated from many plants, but there is no literature report on the isolation of natural quinones from Japanese maple poplar

Method used

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  • Pterocarya stenoptera quinone, its preparation and use
  • Pterocarya stenoptera quinone, its preparation and use
  • Pterocarya stenoptera quinone, its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Extraction, separation and purification of compound I

[0033]

[0034] Compound I

[0035] Take the dry stem bark (3.5 kg) of Tokyo Maple Populus and pulverize it, soak it in 10 liters of 60% ethanol at room temperature for 6 days, filter the aqueous ethanol solution, and repeat the same extraction 8 times. The combined extracts were concentrated under reduced pressure to contain no ethanol (about 2 liters), extracted four times with an equal volume of chloroform, the combined chloroform extracts were concentrated and dried to obtain 10.4 g of chloroform extracts.

[0036] Take 10.4 grams of chloroform extract, dissolve it with an appropriate amount of chloroform, add 10 grams of thin-layer chromatography silica gel G to mix the sample, use 194 grams of silica gel H to dry the decompression column, and use chloroform→methanol gradient (100:0→1:1) Separation by elution chromatography yielded 34 fractions. Each fraction was subjecte...

Embodiment 2

[0072] Embodiment 2. The biological activity test of compound I

[0073] Experimental samples and experimental methods

[0074] Preparation of the tested sample solution

[0075] Take the pure compound I isolated and refined in the above Example 1, accurately weigh an appropriate amount, prepare a 10 mg / ml concentrated solution with methanol, and properly dilute as required for activity testing.

[0076] Cell Lines and Cell Culture

[0077] The mouse breast cancer tsFT210 cell line was used for the activity test, and the cells were subcultured in RPMI-1640 medium containing 10% FBS at 32° C. in an incubator filled with 5% carbon dioxide.

[0078] Flow Cytometry Test Method

[0079] Take the tsFT210 cells in the logarithmic growth phase, and use fresh RPMI-1640 medium to prepare a density of 2×10 per ml 5 The cell suspension of each cell was inoculated in a 24-well plate at 0.5 ml per well, and 5 microliters of sample solutions of different concentrations were added to each w...

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Abstract

The invention discloses a Pterocarya stenoptera quinines compounds and method for preparation, theses compounds having apoptosis inducement and propagation suppression actions to tumor cells, thus can be used as cell apoptosis inducer, cell propagation depressant and antineoplastic agent.

Description

technical field [0001] The invention relates to maplequinone (English name Pterocaryquinone) and its preparation method and application. More specifically, the present invention relates to quinone compounds with a new chemical skeleton structure type extracted from Pterocarya tonkinesis (Franch.) Dode. Use of a tumor cell killing agent, a tumor cell proliferation inhibitor or an antineoplastic agent. Background technique [0002] Quinone compounds mainly contain four types of benzoquinone, naphthoquinone, anthraquinone and phenanthrenequinone, and there are also many quinone compounds known so far (refer to literature: Yao Xinsheng, editor-in-chief, Natural Medicinal Chemistry (third edition), People's Health Press, 2002). There are also many reports about the biological activity of quinone compounds, such as literature (Gong Baoan, etc., structural characteristics and antioxidant activity of tanshinone compounds, Acta Biophysics, 2001, 17 (2), 383-388) It has been reporte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00C07C46/10C07C50/38
Inventor 崔承彬刘红兵蔡兵顾谦群赵庆春管华诗
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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