Recombinant human antibody against HBsAg and its preparation method
A surface antigen, human-derived technology, applied in recombinant DNA technology, anti-animal/human immunoglobulin, using vectors to introduce foreign genetic material, etc., can solve the problems of difficult renaturation and low expression
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[0060] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, but the embodiments are only used to illustrate the present invention rather than limit the present invention.
[0061] 1. Obtaining anti-HBsAg Fab antibody fragment gene by phage display technology
[0062] On the fifth day after the booster injection of hepatitis B vaccine volunteers, blood was collected, monocytes were isolated, mRNA was extracted, cDNA was synthesized by reverse transcription, and the light chain gene and heavy chain Fd of the anti-HBsAg antibody were amplified by PCR with the designed primers. The fragment genes were respectively inserted into the corresponding sites of the vector pComb3H (Carlos F.Barbas and Dennis R.Burton, 1994, Cold Spring Harbor Laboratory, Monoclonal Antibodies from combinatorial Libraries) to construct vector plasmids containing antibody light chain and heavy chain Fd segments. Transform the competent...
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