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New transgenic zebra fish, production method of gene segment and transgenic zebra fish

A gene fragment, zebrafish technology, applied in the field of production of new transgenic golden zebrafish, can solve the problems of good acceptance by consumers, no commercial value of decorative fish, no practicality, etc.

Inactive Publication Date: 2005-03-09
TAIKONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its impracticality and various difficulties, previous fluorescent fish species were not well accepted by consumers and did not have commercial value as decorative fish

Method used

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  • New transgenic zebra fish, production method of gene segment and transgenic zebra fish
  • New transgenic zebra fish, production method of gene segment and transgenic zebra fish
  • New transgenic zebra fish, production method of gene segment and transgenic zebra fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] The following examples are non-limiting and merely representative of various aspects and features of the invention.

[0038] Method for producing golden zebrafish with red fluorescence

[0039] 1. Select a commercially available plasmid construct pDsRed2-1 and p-αEGFPITR as basic materials. Plasmid construct pDsRed2-1 can be purchased from Clontech. The plasmid construct p-αEGFPITR can be described according to relevant literature (such as "Constant GFP expression in F0 generation transgenic killifish (medaka, scientific name Oryzias latipes)", Chi-Yuan Chou et al., Transgenic Research 10: 303-315 , 2001) production.

[0040] 2. Cut out the DsRed fragment from the plasmid pDsRED2-1. Then, as depicted in Figure 2, the CMV promoter and two AAV inverted terminal repeats (ITRs) were ligated to the DsRed fragment to generate the plasmid construct pDsRedITR. A greater degree of expression stability was achieved with the plasmid construct pDsRedITR.

[0041] 3. Formation ...

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PUM

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Abstract

A transgenic golden zebra danio, and a method for recombining its DNA and a novel gene fragment for preparing the transgenic golden zebra danio are disclosed.

Description

technical field [0001] The present invention relates to a method for producing a novel transgenic golden zebra danio. The present invention also relates to novel gene fragments and novel transgenic golden zebrafish. Background technique [0002] Decorative fish is part of the aquatic business, which belongs to the entertainment industry with a globalized industry. Therefore, using recombinant DNA and transgenic technology to improve decorative fish can bring good economic benefits. [0003] However, traditional transgenic techniques can only produce transgenic fish expressing mosaic or weak fluorescence. These fish can only be seen under a fluorescence microscope with a specific wavelength. Due to their impracticality and various difficulties, previous fluorescent fish species were not well accepted by consumers and did not have commercial value as decorative fish. Contents of the invention [0004] The aim of the present invention is to apply recombinant DNA technolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027C12N15/11C12N15/12C12N15/63C12N15/87
Inventor 蔡怀桢
Owner TAIKONG