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Usage of honokiol in the preparing process of anti tumor angiopoiesis medicine

A technology of honokiol and anti-tumor, applied in the field of natural compounds, to achieve the effect of good application prospect and low toxicity

Inactive Publication Date: 2005-03-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far there is only one report that honokiol can inhibit angiogenesis, but this report is limited to the blocking effect of honokiol on the phosphorylation of vascular endothelial growth factor (VEGF) type II receptor by honokiol

Method used

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  • Usage of honokiol in the preparing process of anti tumor angiopoiesis medicine
  • Usage of honokiol in the preparing process of anti tumor angiopoiesis medicine
  • Usage of honokiol in the preparing process of anti tumor angiopoiesis medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Honokiol inhibits hypoxia inducible factor-1 (HIF-1) mRNA in tumor cells

[0030] HIF-1 is one of the most critical factors for tumor angiogenesis. When the content of HIF-1 decreases, angiogenesis in tumor tissue will be blocked. On the contrary, blood vessels in tumor tissue will proliferate and promote tumor growth.

[0031] (1) Experimental materials:

[0032] Cell line: Colorectal cancer cell line RKO comes from American Type Culture Collection (ATCC) company.

[0033] Reagents: Honokiol, China Pharmaceutical and Biological Products Laboratory; Reverse Transcription Kit (Promage, USA), Reverse Transcription Polymerase Chain Reaction (RT-PCR) Kit, Thermostable DNA Synthetase (Taq Shanghai Shenggong) .

[0034] Instruments: culture flask, culture plate, carbon dioxide incubator, PCR machine, FCM instrument.

[0035] (2) Experimental method:

[0036] Cell culture: Grouping: Divided into 5 groups according to different honokiol concentrations, represented by 1, ...

Embodiment 2

[0048] Example 2: Honokiol's Inhibition of Hypoxia Inducible Factor-1 (HIF-1) Protein in Tumor Cells

[0049] Example 1 shows that honokiol can inhibit HIF-1 gene transcription, but it has not been proved that honokiol can also decrease the HIF-1 protein content in tumor cells. This example proves that honokiol has this effect.

[0050] (1) Experimental materials:

[0051] Cell line: The colorectal cancer cell line RKO comes from ATCC, USA.

[0052] Reagents: Honokiol, China Pharmaceutical and Biological Products Laboratory; Polyvinylidene fluoride membrane (PVDF) membrane (Bio-Rad), horseradish peroxidase (HRP) labeled goat anti-mouse antibody (KPL, USA ), mouse anti-human HIF-1α (NeoMarkers, USA), chemiluminescence detection system ECL reaction solution (SantaCruz company), protein determination kit (DC Protein Assay Kit, Bio-Rad company), immunohistochemistry kit (Fuzhou Mai New company).

[0053] Equipment: culture flask, culture plate, carbon dioxide incubator, electrophoresi...

Embodiment 3

[0062] Example 3: The effect of honokiol on the expression of vascular growth factor (VEGF) mRNA in RKO cells

[0063] HIF-1 can up-regulate the vascular growth factor VEGF, which stimulates tumor angiogenesis. Honokiol can down-regulate HIF-1, HIF-1 in tumor cells decreases, and VEGF should also decrease. Based on this reasoning, when honokiol treats tumor cells, VEGF should also be down-regulated.

[0064] (1) Experimental materials

[0065] Cells: The colorectal cancer cell line RKO comes from ATCC, USA.

[0066] Reagents: Reverse transcription kits and PCR kits are products of Promega, USA. Honokiol, China Pharmaceutical and Biological Products Laboratory.

[0067] Instrument: PCR machine.

[0068] (2) Experimental method

[0069] Drug induction: RKO cells 2×10 5 Inoculate in a 6-well plate, culture with RPIM1640 medium containing 10% calf serum for 24 hours to make it adhere to the wall; dilute with RPIM1640 medium containing 3% calf serum and magnolol stock solution (5mg / ml)...

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Abstract

The invention provides the use of honokiol in the preparing process of anti tumor angiopoiesis medicine with a CAS number of 35354-74-6, the molecular weight being 266.33, the honokiol medicine comprises preparation allowed pharmaceutical excipient or carrier. The dosage form can be intestinal tract or non-intestinal tract combination, and the preparation can be made into liquid preparation, granule, tablet, electuary, capsule, drop pill or injection.

Description

Technical field [0001] The invention belongs to the use of natural compounds, and relates to the new use of honokiol extracted from plants, in particular to the use of honokiol in the preparation of anti-tumor angiogenesis drugs. Background technique [0002] In recent years, the issue of angiogenesis in tumor growth has received increasing attention, and some evidences about tumor angiogenesis dependence have been accumulated, and angiogenesis has been found to be controlled by a series of positive and negative regulatory factors. Normal cells are nonangiogenic, and the phenotypic transition from non-angiogenic to angiogenic must occur in either the precancerous stage or the cancerous transformation stage in order for tumors to grow. Histological observation of the angiogenesis model found that there are several obvious stages of angiogenesis 【1】 : Increased vascular permeability causes extravasation of plasma protein and degradation of surrounding extracellular matrix; endothel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K31/05A61P35/00
Inventor 胡汛陈菲王弢
Owner ZHEJIANG UNIV
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