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New esophagus cancer markgene RPL 14 and its use

A technology for marker genes and esophageal cancer, applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve problems such as unreported relationships

Inactive Publication Date: 2005-05-25
TUMOUR INST TUMOR HOSPITAL CHINA ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the relationship between changes in RPL14 and esophageal cancer has not been reported

Method used

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  • New esophagus cancer markgene RPL 14 and its use

Examples

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Embodiment 1

[0021] Example 1: Method for Detecting RPL14 Gene Deletion

[0022] 1) Extract the peripheral blood of the subject and the DNA of the tissue to be investigated by conventional methods [Molecular Cloning Experiment Guide (Second Edition), J. Sambrook, et al., Cold Spring Harbor Laboratory Press, 1989];

[0023] 2) Use primers R1 and R2 to carry out PCR amplification according to the following conditions:

[0024] R1: 5'AAG CAC CTG GTA CTA AGG GTA 3' (SEQ ID NO: 2)

[0025] R2: 5' TCG CGG CGG TGA TCT TTT TTG 3' (SEQ ID NO: 3)

[0026] PCR reaction system

[0027] Genomic DNA 1.0μl (40ng)

[0028] 10×PCR buffer [100mM Tris-HCl (pH8.3), 500mM KCl] 2.0μl

[0029] 25mM MgCl 2 1.2μl

[0030] 2.5mM dNTP 1.0μl

[0031] Upstream primer (10μM) 1.0μl

[0032] Downstream primer (10μM) 1.0μl

[0033] Deionized formamide 0.5μl

[0034] Taq enzyme (10u / μl) 0.2μl

[0035] Double distilled water 12.1μl

[0036] Total reaction volume 20.0μl

[0...

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Abstract

A novel esophagus cancer marking gene RPL14 shown by the 2.442.386-2.442.81 bp sequence of NT-037563.3 in GenBank or by SEQ ID No.1 is disclosed. It can be used to detect the esophagus cancer, that is, the deficiency of gene RPL14 can be used as the marker of early esophagus cancer, warning esophagus cancer, postjudgement and therapy effect.

Description

field of invention [0001] The present invention relates to the use of an esophageal cancer diagnostic marker gene RPL14 (as shown in the 2,442,386-2,442,814 bp sequence of NT_037565.3 in GenBank or as shown in SEQ ID NO: 1) for detecting esophageal cancer. Specifically, the deletion of the gene RPL14 can be used as an early diagnosis sign of esophageal cancer; as a sign of early warning of esophageal cancer; as a sign of prognosis judgment of esophageal cancer and as a sign of esophageal cancer treatment monitoring, wherein the test sample contains the number of cells with the gene deletion A decrease indicates a good therapeutic effect. Background of the invention [0002] Using molecular biology techniques, it is possible to effectively detect and / or diagnose diseases at the molecular level, thereby facilitating the early treatment of these diseases. [0003] Esophageal cancer is one of the most common digestive tract tumors, and its mortality rate ranks fourth among the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 王明荣徐昕蔡岩黄晓平赵春霞韩亚玲吴旻
Owner TUMOUR INST TUMOR HOSPITAL CHINA ACAD OF MEDICAL SCI