Method of preparing fugutoxin water extract

A technology of tetrodotoxin and water extract, applied in the direction of organic chemistry, can solve problems affecting the precipitation rate of tetrodotoxin, waste of manpower and financial resources, loss of tetrodotoxin, etc., and achieve the effect of facilitating coagulation, avoiding loss, and reducing production costs

Inactive Publication Date: 2005-08-10
赵继红
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Problems solved by technology

The preparation of tetrodotoxin aqueous extract is an important link in the production of tetrodotoxin. The production of known tetrodotoxin aqueous extract adopts the pulverization of puffer fish ovaries or/and liver, and continuous s

Method used

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Embodiment Construction

[0013] Below with 100 kilograms of puffer fish ovum preparation tetrodotoxin aqueous extract is example, a kind of method for preparing tetrodotoxin aqueous extract of the present invention mainly comprises the following steps:

[0014] 1. First, quickly cool the puffer fish eggs, and cut them into flakes without thawing;

[0015] 2. Add 400KG of deionized water to soak, leave it to ferment at 28°C, stir gently every 6 hours, stir for 3 minutes, and leave it to ferment for 36 hours. At this time, the water quality is whitish and flaky Egg masses surface.

[0016] 3. Remove egg masses and ovarian foreskins, leave a small amount of eggs in the feed liquid, heat the feed liquid vigorously, keep boiling for 3 minutes, and quickly cool down to 40-50°C within 10 minutes;

[0017] 4. After boiling and cooling, the feed liquid is in the shape of an egg flower, and the feed liquid is subjected to coarse filtration and fine filtration to obtain tetrodotoxin water extract, which is chil...

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Abstract

A process for preparing the liquid extract of tetrodotoxin includes such steps as quickly freezing the viscera of globefish, cutting to become small blocks, immersing in deionized water, fermenting, quick heating, quick cooling, primary filtering and fine filtering. It has high output rate.

Description

technical field [0001] The invention relates to extracting substances from animal materials, in particular to a method for preparing tetrodotoxin aqueous extract from puffer fish viscera. Background technique [0002] Tetrodotoxin is a highly toxic alkaloid extracted from the viscera of poisonous puffer fish, which can specifically block the nerve cell membrane Na + It is highly effective for analgesia and detoxification. The content of tetrodotoxin in puffer fish viscera is low. According to domestic and foreign literature reports, only about 1 gram of tetrodotoxin can be extracted per 100 kg of puffer fish viscera. The output of tetrodotoxin is very small, which seriously restricts its new drug clinical application. The preparation of tetrodotoxin aqueous extract is an important link in the production of tetrodotoxin. The production of known tetrodotoxin aqueous extract adopts the pulverization of puffer fish ovaries or / and liver, and continuous stirring at low temperatur...

Claims

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Application Information

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IPC IPC(8): C07D491/22
Inventor 赵继红
Owner 赵继红
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