Method of preparing fugutoxin water extract

Abstract

A process for preparing the liquid extract of tetrodotoxin includes such steps as quickly freezing the viscera of globefish, cutting to become small blocks, immersing in deionized water, fermenting, quick heating, quick cooling, primary filtering and fine filtering. It has high output rate.

Description

technical field

[0001] The invention relates to extracting substances from animal materials, in particular to a method for preparing tetrodotoxin aqueous extract from puffer fish viscera. Background technique

[0002] Tetrodotoxin is a highly toxic alkaloid extracted from the viscera of poisonous puffer fish, which can specifically block the nerve cell membrane Na + It is highly effective for analgesia and detoxification. The content of tetrodotoxin in puffer fish viscera is low. According to domestic and foreign literature reports, only about 1 gram of tetrodotoxin can be extracted per 100 kg of puffer fish viscera. The output of tetrodotoxin is very small, which seriously restricts its new drug clinical application. The preparation of tetrodotoxin aqueous extract is an important link in the production of tetrodotoxin. The production of known tetrodotoxin aqueous extract adopts the pulverization of puffer fish ovaries or / and liver, and continuous stirring at low temperatur...