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Method of detecting tuberculosis mycobacillus complex and non tuberculosis mycobacillus and its reagent box

A technology for mycobacterium tuberculosis and mycobacterium, applied in the field of molecular biology and nucleic acid detection

Inactive Publication Date: 2006-01-25
SHANGHAI XINGYAO MED TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, it is difficult to quickly and easily detect and identify Mycobacterium tuberculosis complex and non-tuberculous mycobacteria simultaneously

Method used

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  • Method of detecting tuberculosis mycobacillus complex and non tuberculosis mycobacillus and its reagent box
  • Method of detecting tuberculosis mycobacillus complex and non tuberculosis mycobacillus and its reagent box
  • Method of detecting tuberculosis mycobacillus complex and non tuberculosis mycobacillus and its reagent box

Examples

Experimental program
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Embodiment 1

[0046] 1 strain source

[0047] Mycobacteria in this study include: M.diernhoferi, M.scrofulaceum, M.gordonae, M.smegmatis, yellow M.flavescens, M.fortuitum, M.intracellulare, M.szulgai, M.avium, Mycobacterium tuberculosis ( M. tuberculosis), M gastri, M. xenopi, M. kansasii, M triviale, M. terrae, abscess Mycobacterium (M.abscessus), colorless mycobacterium (M.nonchromogenic), provided by China Institute of Pharmaceutical and Biological Products. Sputum samples from clinical tuberculosis patients were provided by Shanghai Pulmonary Hospital.

[0048] 2 Processing of samples

[0049] Various mycobacterial cultures or treated clinical samples were suspended in 10 mM TE (pH 8.0), centrifuged at 15Krpm for 5 minutes, and the supernatant was discarded.

[0050] Add twice the volume of 4% NaOH solution to the sputum samples of clinical tuberculosis patients and healthy people, pipette evenly, and let it stand at room temperature for 30 minutes; then take 1ml of liquefied sputum...

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Abstract

The invention discloses a method to rapidly check out the nodule embranchment bacilli and the non-nodule embranchment bacilli. It includes the following steps: under the condition of amplifying specificity, taking polymerase chain reaction in polymerase chain system. The invention supplies the corresponding agent box. It could rapidly and easily check out and identify the nodule embranchment bacilli and the non-nodule embranchment bacilli.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and nucleic acid detection. More specifically, it relates to a method and kit for rapid detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria. Background technique [0002] Mycobacterium tuberculosis can invade many organs, and the most common form of pulmonary tuberculosis is pulmonary involvement, which is an infectious disease. Excretion patients are its important source of infection. The basic pathological features of this disease are exudation, caseous necrosis, and other proliferative tissue reactions, which can form cavities. Clinically, it is mostly a chronic process, manifested as cough, hemoptysis, low-grade fever, weight loss, and fatigue. If timely diagnosis and reasonable treatment, most of them can be clinically cured. The control of the tuberculosis epidemic in various regions in my country is uneven, and it is currently one of the top ten c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/34C12Q1/68
Inventor 周煜夏懿
Owner SHANGHAI XINGYAO MED TECH DEV CO LTD
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