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Method for detecting tetracycline through semiquantitative test paper of immunity chromatography

A detection method and immunochromatographic technology, which are applied in the field of rapid detection of tetracycline immunochromatography, can solve the problems of incomplete tetracycline and large toxic and side effects of residues.

Inactive Publication Date: 2006-02-01
SOUTH CHINA AGRI UNIV
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Problems solved by technology

Tetracycline is an antibiotic extracted from the fermentation broth of Chrysoderma fungus, and its hydrochloride is generally used, which plays an important role in the treatment of livestock Gram-positive and negative bacteria, rickettsia, spirochetes and some protozoa infectious diseases effect, but its residues have relatively large toxic and side effects
Therefore, the corresponding tetracycline detection technology in my country is not very complete at present, and it is necessary to study a simpler, faster and more convenient method

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  • Method for detecting tetracycline through semiquantitative test paper of immunity chromatography

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Experimental program
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Embodiment Construction

[0023] (1) Test paper in the form of 1 detection line + 1 reference line

[0024] The detection line is coated with a tetracycline monoclonal antibody or an antigen for tetracycline detection at a concentration of 0.1-30 μg / mL, with a width of 1 mm; the reference line is coated with an IgG against the second species of animal protein at a concentration of 0.01-30 μg / mL 1 line, 1mm wide; the interval between two lines is 2-6mm. When the color of the detection line is the same as that of the reference line, the sample is negative; when the color of the detection line is lighter than the reference line, the sample is positive, and the concentration is greater than Aμg / kg, A is the set detection limit, which can be greater than or equal to A certain concentration of 10 μg / kg. The reference line is always in color, if the reference line does not show color, it indicates that the test paper is invalid.

[0025] (2) Test paper in the form of 2 detection lines + 1 reference line

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Abstract

A method of using immune chromatography semi ¿C quantitative test paper to detect tetracycline sticks sample solution absorption portion, colloidal gold labeled portion, detecting reaction portion and water absorption portion in sequence on backing of test paper . The detecting reaction portion is prepared as coating 1 ¿C 3 bar of tetracycline antibody or 1 ¿C 3 bar of detection antigen as detection line and coating 1 ¿C 3 bar of IgG for anti ¿C second generic animal protein as reference line, and using 2 ¿C 4 bar of combination line formed by said two lines being used no more than one bar at the same time in combination.

Description

technical field [0001] The invention relates to a method for detecting antibiotic residues in animal food, in particular to a method for rapidly detecting tetracycline immunochromatography. Background technique [0002] Antibiotic residue is a common problem in livestock and poultry farming all over the world, among which tetracycline is a relatively common residual antibiotic. Tetracycline is an antibiotic extracted from the fermentation broth of Chrysoderma fungus, and its hydrochloride is generally used, which plays an important role in the treatment of livestock Gram-positive and negative bacteria, rickettsia, spirochetes and some protozoa infectious diseases effect, but its residues are relatively toxic and side effects. In order to strengthen the management of veterinary drug residues and ensure the quality and safety of animal products, the Ministry of Agriculture of my country revised the "Maximum Residue Limits of Veterinary Drugs in Animal Fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543G01N21/78G01N33/52
Inventor 雷红涛孙远明黄晓钰吴青王弘谌国莲
Owner SOUTH CHINA AGRI UNIV
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