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Method for detecting gentamycin through semiquantitative test paper of immunity chromatography

A technology for detection of gentamicin and its detection method, which is applied in the field of detection of antibiotic residues and can solve problems such as incomplete detection technology of gentamicin

Inactive Publication Date: 2006-02-01
SOUTH CHINA AGRI UNIV
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  • Description
  • Claims
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Problems solved by technology

Therefore, the corresponding gentamicin detection technology in my country is not very complete at present, and it is necessary to study a simpler, faster and more convenient detection method

Method used

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  • Method for detecting gentamycin through semiquantitative test paper of immunity chromatography

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Experimental program
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Embodiment Construction

[0023] (1) Test paper in the form of 1 detection line + 1 reference line

[0024] The detection line is coated with gentamicin monoclonal antibody or gentamicin detection antigen with a concentration of 0.1-30 μg / mL, and the width is 1 mm; the reference line is coated with anti-secondary antibody with a concentration of 0.01-30 One IgG line of animal protein, 1mm wide; the interval between two lines is 2-6mm. When the color of the detection line is the same as that of the reference line, the sample is negative; when the color of the detection line is lighter than the reference line, the sample is positive, and the concentration is greater than Aμg / kg, A is the set detection limit, which can be greater than or equal to A certain concentration of 10 μg / kg. The reference line is always in color, if the reference line does not show color, it indicates that the test paper is invalid.

[0025] (2) Test paper in the form of 2 detection lines + 1 reference line

[0026] 2 detection...

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Abstract

A method of using immune chromatography semi ¿C quantitative test paper to detect gentamicin sticks sample solution absorbing portion, colloidal gold labeled portion, detecting reaction portion and water absorbing portion in sequence on backing of test paper. The detecting reaction portion is prepared as coating 1 ¿C 3 bar of gentamicin antibody or detection antigen as detection line, coating 1 ¿C 3 bar of IgG for anti ¿C second generic animal protein as reference line and using 2 ¿C 4 bar of combination line formed by said two lines being used no more than one bar at the same time in combination.

Description

technical field [0001] The invention relates to a method for detecting antibiotic residues in food, in particular to a method for detecting gentamicin semi-quantitative test paper. Background technique [0002] Antibiotic residue is a common problem in livestock and poultry farming all over the world, and gentamicin is a relatively common residual antibiotic. Gentamicin is an aminoglycoside antibiotic, which plays an important role in the treatment of infectious diseases of Pseudomonas aeruginosa, Proteus and penicillin-resistant Staphylococcus aureus in livestock, but its residues are relatively toxic and side effects. In order to strengthen the management of veterinary drug residues and ensure the quality and safety of animal products, the Ministry of Agriculture of my country revised the "Maximum Residue Limits of Veterinary Drugs in Animal Foods" in 2002, which stipulates that the maximum residue limit of gentamicin in milk is 200 μg / kg, which is 20...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543G01N21/78G01N33/52
Inventor 雷红涛孙远明黄晓钰王弘潘科吴青谌国莲
Owner SOUTH CHINA AGRI UNIV