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Process for preparing high-immune serum for main epidemic disease of dog and formulation thereof

A high-immune serum and preparation technology, which is applied in the direction of antiviral agents, immunoglobulins derived from serum, and medical raw materials derived from mammals, etc., can solve the problems of heterologous protein allergy, complicated process, hidden safety hazards, etc., and reach the shelf life Long, simple production process, easy to preserve the effect

Inactive Publication Date: 2006-02-08
MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some people in my country have developed anti-virus immunoglobulin preparations against dogs, raccoon dogs, foxes and cats, but they are all prepared from immune equine animals, and the Fc fragment of the immunoglobulin must be removed after enzymatic digestion, otherwise it is easy to cause heterologous protein allergies, complex
In addition, during the preparation process of blood products, due to the replication of viruses or other viral diseases in animals, blood may be contaminated with some viruses. If not properly handled, it will bring safety hazards to the use of blood product preparations. Therefore, There must be proper virus inactivation process steps in the preparation process of blood products, but for this reason, no virus removal and inactivation technology has been applied to production in the preparation process of animal blood products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Anti-canine distemper, canine parainfluenza, canine infectious hepatitis, canine infectious laryngotracheitis and canine parvovirus enteritis combined hyperimmune serum preparation prepared from dogs

[0024] 1. Immunogen Preparation

[0025] Inoculate MDCK cells with canine distemper virus, canine parainfluenza virus, canine adenovirus type I and type II, inoculate canine parvovirus into F81 cells, and culture in spinner bottles at 37°C. Canine distemper virus, canine adenovirus and canine parainfluenza virus were cultured for 3-5 days to collect the virus, and canine parvovirus was cultured for 2-3 days to collect the virus. After repeated freezing and thawing, the cell debris was removed by centrifugation, and the virus titer was measured by sampling. Dilute the 5 kinds of virus liquids until the virus titers are all greater than 10 4.0 TCID 50 / 0.01mL, mix well, add aluminum glue or Freund's adjuvant according to the ratio of virus solution:adjuvant=1-9:1, make im...

Embodiment 2

[0048] Anti-canine distemper, canine parainfluenza, canine infectious hepatitis, canine infectious laryngotracheitis and canine parvovirus enteritis combined hyperimmune serum preparation prepared from foxes

[0049] 1. Immunogen Preparation

[0050] Inoculate MDCK cells with canine distemper virus, canine parainfluenza virus, canine adenovirus type I and type II, inoculate canine parvovirus into F81 cells, and culture in spinner bottles at 37°C. Canine distemper virus, canine adenovirus and canine parainfluenza virus were cultured for 3-5 days to collect the virus, and canine parvovirus was cultured for 2-3 days to collect the virus. After repeated freezing and thawing, the cell debris was removed by centrifugation, and the virus titer was measured by sampling. Dilute the 5 kinds of virus liquids until the virus titers are all greater than 10 4.0 TCID 50 / 0.01mL, mix well, add aluminum glue or Freund's adjuvant according to the ratio of virus solution:adjuvant=1-9:1, make i...

Embodiment 3

[0073] Anti-canine distemper, canine parainfluenza, canine infectious hepatitis, canine infectious laryngotracheitis and canine parvovirus enteritis combined hyperimmune serum preparation prepared from raccoon dog

[0074] 1. Immunogen Preparation

[0075] Inoculate MDCK cells with canine distemper virus, canine parainfluenza virus, canine adenovirus type I and type II, inoculate canine parvovirus into F81 cells, and culture in spinner bottles at 37°C. Canine distemper virus, canine adenovirus and canine parainfluenza virus were cultured for 3-5 days to collect the virus, and canine parvovirus was cultured for 2-3 days to collect the virus. After repeated freezing and thawing, the cell debris was removed by centrifugation, and the virus titer was measured by sampling. Dilute the 5 kinds of virus liquids until the virus titers are all greater than 10 4.0 TCID 50 / 0.01mL, mix well, add aluminum glue or Freund's adjuvant according to the ratio of virus solution:adjuvant=1-9:1, ...

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Abstract

The invention provides a process for preparing high-immune serum for main epidemic disease of dog and formulation, which comprises subjecting one or several pathogens causing serious diseases of canine animals to culture and propagation of primary or passage cell or bacteria culture media of dogs, monkeys and cats, charging adjuvant to obtain immunogens, immuning the canine animals and separating serum, eradicating virus, finally making single or combined serum or antibody preparation.

Description

Technical field: [0001] The present invention relates to the preparation process of hyperimmune serum against important canine diseases of canines, and in particular provides a preparation process and preparation of hyperimmune serum of canines against important canine diseases, which is used for emergency prevention of corresponding important diseases of canines and treatment, belonging to the field of biological product development. Background technique: [0002] Along with dog, raccoon dog, fox and other canine fur animal breeding industry develops continuously in our country, the number of fur animal breeding reaches several million every year. However, many important diseases of canine animals such as canine distemper, canine parvovirus disease, canine infectious hepatitis (fox encephalitis), canine infectious laryngotracheitis, canine influenza, canine parainfluenza, canine coronavirus disease, canine leptovirus The continuous occurrence of epidemic diseases such as s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/16C07K16/06A61K39/395A61P31/12
Inventor 夏咸柱黄耕王承宇高玉伟杨松涛
Owner MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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