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Method for preparing lactate biosensing strip

A sensing strip, biological technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2006-03-01
COUNCIL OF SCI & IND RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another disadvantage is that the detection of lactate levels using the above-mentioned prior art methods needs to be done in a laboratory by qualified personnel

Method used

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  • Method for preparing lactate biosensing strip
  • Method for preparing lactate biosensing strip
  • Method for preparing lactate biosensing strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1: the preparation of the graphite paste that contains medium

[0070] 100 mg of graphite powder and polyvinylpyrroline (binder) were mixed with 0.01 M potassium ferricyanide (medium) in ethylene glycol monobutyl ether to prepare a screen-printable graphite paste for the working electrode.

Embodiment 2

[0071] Example 2: Preparation of Dry Bars

[0072] A commercially available lactate oxidase solution (2 μl) containing 2 U lactate oxidase was physically absorbed on a sensor strip mixed with a graphite electrode medium and kept at 25° C. overnight to dry. The dried electrode was coated with a layer of hydrophilic nylon membrane. Before the film was applied, it was placed in distilled water containing 10% surfactant (Tween 80) for a period of time and the dried film was fixed on the strip.

Embodiment 3

[0073] Embodiment 3: the preparation of lactic acid standard lactic acid solution

[0074] Prepare a 10 mM stock lactic acid solution in 0.1 M phosphate buffer. The stock solution was diluted with phosphate buffer to obtain 2mM, 4mM, 6mM and 8mM standard solutions.

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Abstract

The present invention provides a method for the manufacture of a lactate biosensing strip for use in the assay of lactate in a sample, said sensor comprising a dry strip sensor of an electrically conducting material having at least: i) an external surface. ii) a screen printed reference electrode and, iii) a screen-printed working electrode.

Description

field of invention [0001] The invention relates to a lactic acid biosensing strip for measuring lactic acid solution. The invention also relates to a manufacturing method of a new lactic acid biosensing strip and its use in lactic acid sensing. Background of the invention [0002] Relying on individual examination and clinical laboratory results, physicians can determine the presence and concentration of biological analytes in critical care patients. In a well-controlled, high-quality environment, clinical laboratories can provide a variety of automated systems to perform high-volume testing and analytical support. However, clinical laboratories do not provide the immediate results needed to treat trauma and multiple organ dysfunction / failure patients. To meet the clinical need for immediate test results, several technologies are emerging through bedside use including electrochemical biosensors, optical fluorescent sensors, paramagnetic particles for coagulation detection ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/00
CPCC12Q1/002C12Q1/005C12Q1/001
Inventor 马诺伊・库马尔・潘迪阿沙・乔贝克里尚・康德・潘德拉金德拉・库马尔・夏尔马克里尚・库马尔・萨伊尼班西・达尔・马尔霍特拉拉杰什
Owner COUNCIL OF SCI & IND RES
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