Exterior guiding gene T6 concurrently used with RnaseP ribozyme and its uses
An external guidance and combination technology, applied in the fields of molecular biology and medicine, can solve the problems of inability to achieve efficient guidance and cutting, difficulties, etc., and achieve the effect of manual operation and control, improve efficiency, and ensure stability and efficiency.
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[0035] Sources of plasmids and strains:
[0036] The vector plasmids pGEM3z, pUC19, and the host Escherichia coli JM109 and DH-5a were purchased from Gene Corporation, and they are commonly used vector plasmids and host bacteria in the field.
[0037] The main reagents are shown in Table 1:
[0038] Endonuclease
Dalian Baosheng Company
T4 DNA Ligase
Dalian Baosheng Company
Mung bean sprout nuclease
Dalian Baosheng Company
T7 RNA polymerase
Gene company
Ex Taq polymerase
Dalian Baosheng Company
RNase inhibitor
Dalian Baosheng Company
RNase-free DNase I
Dalian Baosheng Company
rNTP, bovine serum albumin component V
Beijing Dingguo Company
RNaseA
Beijing Dingguo Company
Tris saturated phenol, water saturated phenol
Beijing Dingguo Company
Electrophoresis grade urea
Beijing Dingguo Company
Wide Range DNA Ladder Marker
Dalian Baosheng Company
Nucl...
PUM
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