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Method for measuring lead in blood by using hydride generating atomic spectrum method

A hydride generation and atomic spectroscopy technology, applied in the field of acid protein precipitation treatment of blood samples, can solve the problems of very strict acidity requirements, long digestion process, low measurement results, etc. Guaranteed effect of stability and reliability

Inactive Publication Date: 2006-08-09
BEIJING JITIAN INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are still some deficiencies in the use of hydride generation-atomic spectrometry to measure blood lead, which mainly focus on two aspects: first, the sample is required to be digested when measuring blood lead by hydride generation-atomic spectrometry, and the digestion process is time-consuming and easy. Pollution, the acid can not clean up; on the other hand, the acidity of the reaction is very strict when the hydride is produced and the blood lead is measured. The available acidity range is less than 0.5% (volume percentage), and a slight deviation will cause the measurement result to be significantly lower.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In the first step of pretreatment of blood samples, add 2.9 ml of 3.5% nitric acid by volume to each of the cleaned centrifuge tubes as the carrier, then add 100 microliters of blood samples, which is equivalent to 30-fold dilution, and take another 4 tubes for cleaning Add 3 ml of 3.5% nitric acid to each of the last centrifuge tubes as a reagent blank, shake and mix all the above centrifuge tubes on a vortex mixer for 30 seconds, let stand for 10 minutes, and then set aside at 3000 rpm Separately centrifuge for 10 minutes, after the centrifugation is completed, a clear and transparent sample solution to be tested is obtained, and the centrifuge tube is taken out and placed on the sample rack for testing;

[0028] The establishment of the standard curve in the second step is that the lead standard solution (GBW08611) of 1000 mg / liter is diluted step by step with 3.5% nitric acid by volume percentage to 10 micrograms / liter, and then 0, 2, 4, 8, 10 micrograms / liter are ta...

Embodiment 2

[0038] In the first step of blood sample pretreatment, add 2.95 ml of hydrochloric acid with a volume percentage of 4.5% to each cleaned centrifuge tube as the carrier, then add 50 microliters of blood sample, which is equivalent to a 60-fold dilution, and take another 4 tubes for cleaning Add 3 ml of 4.5% hydrochloric acid by volume to each of the last centrifuge tubes, as a reagent blank, vibrate and mix all the above centrifuge tubes on an ultrasonic oscillator for 60 seconds, then let stand for 2 minutes, and then rotate at 10,000 rpm Separately centrifuge for 5 minutes, after the centrifugation is completed, a clear and transparent sample solution to be tested is obtained, and the centrifuge tube is taken out and placed on the sample rack for testing;

[0039] The establishment of the second step standard curve is that the lead standard solution (GBW08611) of 1000 mg / liter is gradually diluted to 10 micrograms / liter with 4.5% hydrochloric acid by volume percentage, and the...

Embodiment 3

[0044] The first step of pretreatment of blood samples is to add 2.95 ml of perchloric acid with a volume percentage of 2% in each cleaned centrifuge tube as the carrier flow, and then add 50 microliters of blood samples, which is equivalent to a 60-fold dilution, and another 4 Add 3 ml of 2% perchloric acid by volume to each cleaned centrifuge tube as a reagent blank, vibrate and mix all the above centrifuge tubes on an ultrasonic oscillator for 50 seconds, let stand for 4 minutes, and then Centrifuge at 8000 rpm for 7 minutes. After the centrifugation is complete, a clear and transparent sample solution to be tested is obtained. Take out the centrifuge tube and place it on the sample rack for testing;

[0045] The establishment of the standard curve in the second step is that the lead standard solution (GBW08611) of 1000 mg / liter is diluted step by step with 2% perchloric acid by volume percentage to 10 micrograms / liter, and then 0, 2, 4, 8, 10 micrograms are taken Five poin...

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Abstract

Present invention relates to method for determining blood specimen lead content by hydride generation-atomic spectrum method. It contains 1, haemoid pretreatment, loading flow in centrifuge tube then adding blood sample and oscillating to mix uniformly, after centrifuging after resting for 2-10 minute to obtain liquid to be measured, 2, establishing standard curve, diluting step by step 1000 milligrams / liter to 10 microgram / litre, determining by hydride generation-atomic spectrum method to obtain lead standard curve, 3, determining sample, determining sample liquid using hydride generation-atomic spectrum method and comparing with standard series quantify. Said method has simple operation, fine stability and reliability, high determination data repetitiveness and accuracy with low cost.

Description

technical field [0001] The invention provides a method for treating blood samples with an acid protein precipitation method, and then using hydride generation-atomic spectrometry to determine the lead content in the blood samples. Background technique [0002] Lead is a well-known toxic element, which can cause damage to the digestive tract, hematopoietic system and nervous system, and is very harmful to the human body (especially children). But at the same time, lead is widely used in storage batteries, gasoline anti-vibration agents, alloys, radioactive protection and other fields, and it cannot be completely eliminated in the short term. This has caused relatively serious lead poisoning, especially for children in the growth and development stage, the proportion of lead poisoning is very high, which is likely to endanger the overall quality of the population in our country. The prevention and treatment of lead poisoning includes primary prevention (stop using leaded gaso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/71G01N33/48
Inventor 刘霁欣陈亨秦德元闫军陈红军刘明钟
Owner BEIJING JITIAN INSTR CO LTD