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Ultraviolet auxiliary extraction and hydride generation-atomic fluorescence spectrometry method for determining lead content in blood

A technology of hydride generation and assisted extraction, which is applied in the direction of biological testing, preparation of test samples, material stimulation analysis, etc., can solve the problems of very strict acidity requirements, long digestion process, and acid cannot be cleaned up, so as to avoid rushing Effects of incomplete acidity, expanded range of available acidity, and cheap consumables

Inactive Publication Date: 2009-09-16
BEIJING JITIAN INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are still some deficiencies in the use of hydride generation-atomic spectrometry to measure blood lead, which mainly focus on two aspects: first, the sample is required to be digested when the hydride generation-atomic spectrometry is used to measure blood lead, and the digestion process is time-consuming and easy. Pollution, the acid also can't clean up; on the other hand, the acidity requirement of the reaction is very strict when the hydride is produced and the blood lead is measured, and the available acidity range is less than 0.5% (volume percentage), and a slight deviation will cause the measurement result to be significantly low; Third, there are often some organic complexing agents in the blood, which will interfere with the hydride generation process of lead, making the measurement results significantly lower

Method used

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  • Ultraviolet auxiliary extraction and hydride generation-atomic fluorescence spectrometry method for determining lead content in blood
  • Ultraviolet auxiliary extraction and hydride generation-atomic fluorescence spectrometry method for determining lead content in blood
  • Ultraviolet auxiliary extraction and hydride generation-atomic fluorescence spectrometry method for determining lead content in blood

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Experimental program
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Effect test

Embodiment 1

[0043] In the first step of blood sample pretreatment, add 2.9 ml of carrier fluid to each of the cleaned centrifuge tubes. The carrier fluid contains 3.5% nitric acid by volume and 1.0% H by volume. 2 o 2 , and then add 100 microliters of blood samples containing the organic complexing agent iminodiacetic acid (IDA), which is equivalent to 30-fold dilution. In addition, take 4 cleaned centrifuge tubes and add 3 milliliters of 3.5% nitric acid in each , as a reagent blank, shake and mix all the above centrifuge tubes on a vortex mixer for 30 seconds, let stand for 10 minutes, and then centrifuge at 3000 rpm for 10 minutes. After centrifugation, put the centrifuge tubes into a static UV-assisted Process in the extractor for 5 to 20 minutes to obtain a clear and transparent sample solution to be tested, take out the centrifuge tube and place it on the sample rack for testing;

[0044] The establishment of the standard curve in the second step is that the lead standard solution ...

Embodiment 2

[0058] For the pretreatment of the first step blood sample, 2.95 ml of 4.5% hydrochloric acid by volume is added to each of the cleaned centrifuge tubes as the carrier, and then 50 microliters of hydrochloric acid containing the organic complexing agent triacetoxyammonia (NTA) is added. Blood sample, equivalent to 60-fold dilution, take 4 additional centrifuge tubes after cleaning, add 3 ml of hydrochloric acid with a volume percentage of 4.5% to each tube, as a reagent blank, shake and mix all the above centrifuge tubes on an ultrasonic oscillator for 60 Seconds, let it stand for another 2 minutes, and then centrifuge at 10,000 rpm for 5 minutes. After the centrifugation is completed, put the centrifuge tube into a dynamic ultraviolet-assisted extraction device for 5 to 20 minutes to obtain a clear and transparent sample solution to be tested. Take out the centrifuge tube and put it on the sample rack to be tested;

[0059] The establishment of the second step standard curve ...

Embodiment 3

[0065] In the pretreatment of the first step blood sample, in each cleaned centrifuge tube, add 2.95 ml of perchloric acid with a volume percentage of 2% as the carrier flow, and then add 50 microliters of organic complexing agent triacetylammonia (NTA ) blood sample, which is equivalent to 60-fold dilution. In addition, take 4 cleaned centrifuge tubes, and add 3 ml of perchloric acid with a volume percentage of 2% to each tube, as a reagent blank. Shake and mix for 50 seconds, then let it stand for 4 minutes, and then centrifuge at 8000 rpm for 7 minutes. After the centrifugation is completed, put the centrifuge tube into a dynamic ultraviolet assisted extraction device for 5 to 20 minutes to obtain a clear and transparent sample. To measure the liquid, take out the centrifuge tube and put it on the sample rack to be tested;

[0066] The establishment of the second step standard curve, the lead standard solution (GBW08619) of 1000 mg / ml is diluted step by step with 2% perchlo...

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Abstract

The invention relates to a method for measuring blood lead by ultraviolet-assisted extraction of hydride generation-atomic spectrometry and an ultraviolet-assisted extraction instrument. The method includes the first step of blood sample pretreatment, the second step of establishing a standard curve and the third step of sample determination. The first step is to oscillate and mix the loading flow with the blood sample, and after centrifugation, put the centrifuge tube into the ultraviolet assisted extraction device for processing; the second step is to obtain the standard curve of lead by using hydride generation atomic spectrometry; the third step is to load The flow carries the sample to be tested to the reaction block and reacts with the reducing agent to generate plumber, which is then sent to the atomizer by the carrier gas for atomization, and the corresponding spectral signal is measured by atomic spectroscopy, which is compared with the standard curve for quantification. The ultraviolet assisted extraction instrument includes a light-tight casing, and more than one ultraviolet lamp is arranged in the casing, and a container for storing samples is arranged on one side or around the lamp. The method makes the measured data more stable and reliable, the consumables are cheap, the method is accurate, fast and convenient, and is very suitable for popularization in primary medical units.

Description

technical field [0001] The invention relates to a method for extracting and treating blood samples assisted by ultraviolet rays, and then using hydride generation atomic spectrometry to determine the lead content in the blood samples. Background technique [0002] Lead is a well-known toxic element, which can cause damage to the digestive tract, hematopoietic system and nervous system, and is very harmful to the human body (especially children). But at the same time, lead is widely used in storage batteries, gasoline anti-vibration agents, alloys, radioactive protection and other fields, and it cannot be completely eliminated in the short term. This has caused relatively serious lead poisoning, especially for children in the growth and development stage, the proportion of lead poisoning is very high, which is likely to endanger the overall quality of the population in our country. The prevention and treatment of lead poisoning includes primary prevention (stop using leaded ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/84G01N1/34G01N21/62
Inventor 刘霁欣秦德元陈志新裴晓华
Owner BEIJING JITIAN INSTR CO LTD