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Synthetic peptide targeting critical sites on the SARS-associated coronavirus spike protein responsible for viral infection and method of use thereof

A technology of use and composition, applied to a synthetic peptide targeting key sites on the SARS-related coronavirus spike protein that causes viral infection, and the field of use thereof, and can solve problems such as increased virus mutation rate

Active Publication Date: 2012-05-02
THE UNIVERSITY OF HONG KONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mutation rates will increase in interspecies transferred viruses due to new selective pressures in new hosts

Method used

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  • Synthetic peptide targeting critical sites on the SARS-associated coronavirus spike protein responsible for viral infection and method of use thereof
  • Synthetic peptide targeting critical sites on the SARS-associated coronavirus spike protein responsible for viral infection and method of use thereof
  • Synthetic peptide targeting critical sites on the SARS-associated coronavirus spike protein responsible for viral infection and method of use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0102] Experimental part

[0103] Cell Cultures and Virus Strains

[0104] The above peptides were tested in fetal rhesus monkey kidney (FRhK-4) cell cultures as described below.

[0105] In MEM medium (10%-MEM, Invitrogen, USA) containing 10% fetal bovine serum at 37°C, 5% CO 2 Fetal rhesus monkey kidney (FRhK-4) cells were cultured and maintained. The SARS-CoV strain GZ50 was isolated from nasopharyngeal washes from a SARS patient in Guangzhou, China in February 2003 and maintained in FRhK-4 cells (13, 14). Serial passage of the GZ50 strain in FRhK-4 cells persistently produced cytopathic effects (CPEs) at a titer of 10 7 TCID50 / ml. Genome sequencing (Accession No. AY304495) and phylogenetic analysis revealed that GZ50 lies between the reported Hong Kong strains and the Canadian and American strains (15).

[0106] Peptide vaccination and virus infection

[0107] About 5,000 FRhK-4 cells were seeded per well in 10%-MEM on a 96-well plate and cultured overnight. ...

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Abstract

The present invention provides methods for locating critical portions or sites on the spike protein (S protein) of SARS-associated coronavirus (SARS-CoV) responsible for the viral infection that causes Severe Acute Respiratory Syndrome (SARS). The present invention also provides new synthetic peptides targeting such critical portions or sites of the S protein of SARS-CoV for preventing or treating of SARS-CoV infection in a subject. The present invention further provides methods of testing antiviral activity exerted by antiviral agents using real-time quantitative PCR.

Description

[0001] This application is a continuation-in-part of US Serial No. 60 / 630,334 filed November 22, 2004, and claims the benefit of priority thereof, the contents of which are hereby incorporated by reference. [0002] Throughout this application, some publications are referenced using parenthetical Arabic numerals. All references cited by parenthetical Arabic numerals can be found at the end of the specification, before the claims. The disclosures of these references in their entireties are incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains. technical field [0003] This application relates to locating and targeting critical parts or sites on the spike protein ("S protein") of SARS-associated coronavirus ("SARS-CoV") that cause severe Viral infection of acute respiratory syndrome (Severe Acute Respiratory Syndrome, "SARS"). The present invention also relates to novel synthetic peptides for the p...

Claims

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Application Information

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IPC IPC(8): G01N33/569C07K14/165G01N33/68C12Q1/70A61P31/14A61P11/00
CPCG01N33/6878G01N2333/165A61K38/00G01N33/56983C12N2770/20022C12Q1/701C07K14/005A61P11/00A61P31/14
Inventor 郑伯建管轶黄建东何明亮
Owner THE UNIVERSITY OF HONG KONG
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