Method for establishing SARS related coronavirus infected mouse model

Abstract

The invention discloses a method for building model mice of the coronavirus related to SARS. Said invention selects MHV-3 or MHV-A59 virus of 10-1000 pFu to infect little mice of Balb/cJ inbred line system by respiratory tract infection, and observes the survival rate of the little mice infected and their histology change and the virus titers change in immune organs as spleen, liver and lung. Sequentially a little model mice of the coronavirus related to SARS, is established as human severe acute respiratory syndrome. The establishment of said model provides a powerful means for systematic research on pathological change, mechanism of cyclosporin, and the functions of epigenetic regulation.

Description

technical field [0001] The invention relates to a method for establishing a SARS-associated coronavirus mouse model, which is used for medical researchers to simulate, study and prevent and treat SARS diseases. Background technique [0002] SARS is a new type of infectious disease that recently appeared in my country and even in the world. my country calls it atypical pneumonia. Since the first case was reported in Foshan City, Guangdong, as of June 1, 2003, excluding Hong Kong and Taiwan, a total of 12 provinces in my country have reported SARS cases, with 5,328 confirmed cases and 332 deaths . Worldwide, the incidence area involves 33 countries and regions, with 8360 confirmed cases and 764 deaths. The speed of the spread of the disease, the wide range involved, and the great degree of harm have shocked the world. Due to the severe epidemic situation in our country, SARS has been listed as a Class I infectious disease. [0003] The current etiology...

AI Technical Summary

Problems solved by technology

However, the sources of non-human primates are scarce, expensive, long breeding cycle, feeding and management are difficult, and their biological safety protection requirements in SARS research have reached the BSL-4 level, that is, the laboratory hardware requirements are extremely high, which severely limits this research. Model popularization and application

[0006] Scientists are still trying to find a more economical and easier-to-obtain model animal to replace the above-mentioned model, but because the coronavirus is highly species-specific, it is more difficult to find an animal model, such as the research conducted by the Chinese Center for Disease Control and Prevention People have tried to establish a SARS model in suckling mice, but failed to achieve satisfactory results

At the same time, scientists found that pet cats in the homes of some SARS-infected residents in Hong Kong carried SARS virus. Therefore, some research units, including the US Centers for Disease Control and Prevention, are using cats to conduct research on SARS virus, although cats are sensitive to mechanical and chemical stimuli. Sex is beneficial to the establishment of respiratory disease models, but it is difficult to raise and reproduce the animals in groups, and psychological abnormalities are prone to occur during the estrus period, so the operability is poor

It was also reported that researchers at the National Laboratory of Microbiology in Winnipeg, Canada detected that pigs and chickens could also be infected by the SARS virus, but the former was large in size, which was not conducive to experimental processing, and the latter was anatomically far from humans. And the lack of clear genetic background data also limits their research on the mechanism of SARS

[0007] After encountering many difficulties in the selection of SARS animal models, the Institute of Microbiology and Epidemiology of the Academy of Military Medical Sciences plans to use transgenic technology to transfer the gene that is sensitive to SARS virus into experimental mice, so that the experimental mice will be as sensitive to SARS as humans Viruses are sensitive. This idea may be a good way to break through the bottleneck of the SARS model, but the search for sensitive genes may be a long process.

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