Method for raising dairy cattle to improve content of conjugated linoleic acid in milk
A technology of conjugated linoleic acid and dairy cows, which is applied in animal feed, animal feed, additional food elements, etc., can solve the problem that the product only has and does not significantly increase the content, and achieves the reduction of milk fat content, significant application value, and enhanced transformation. Effect
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Embodiment 1
[0016] Embodiment 1, dairy cow is raised
[0017] 1. Screening of cattle for production (from Mengniu Ausia Ranch)
[0018] Breed: Australian Holstein
[0019] Lactation stage: early and late lactation to mid-lactation, that is, 60 to 210 days after delivery
[0020] Milk production: ≥7500kg / year
[0021] Weight: 540±20kg
[0022] CLA background content in milk fat: ≥5.5mg / g milk fat
[0023] Cow age: more than three years old, between three and six lactation periods
[0024] 2. Breeding environment
[0025] 1) Drinking water: Provide sufficient and clean drinking water inside and outside the house, and control the water temperature at 21-27°C; at the same time, properly put coarse materials and mineral salts in the playground;
[0026] 2) Cowshed temperature: 10-18°C;
[0027] 3) Lighting: white fluorescent lamps are used for lighting, so that the day and night are cycled according to the ratio of 16 hours and 8 hours to increase the light;
[0028] 4) Exercise: Stren...
Embodiment 2
[0047] Example 2, the functional experiment of milk containing conjugated linoleic acid of the present invention
[0048] 1. Lipid-lowering efficacy test
[0049] Take the CLA milk of Example 1 as a sample
[0050] 1.1 Test animals
[0051] Male secondary Kunming mice (Yidongzi No. 01-3001), aged three weeks, weighing 12.7±1.0 g, 50 mice. Provided by the Experimental Animal Breeding Farm, Institute of Medical Experimental Animals, Chinese Academy of Medical Sciences. The experimental rats were raised in cages with a humidity of 50-60% and a temperature of 18-22° C., 4 rats per cage, free to eat standard basic pellet feed, and free to drink water.
[0052] 1.2 Dose design
[0053] The recommended dosage for the human body of this sample is 500g / 60kg per day. This experiment did not set up three dosage groups of low, medium and high dosage, but only used the same intake dosage, and the blank control group was fed with ordinary sterilized milk.
[0054] 1.3 Administration of...
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