Composite microorganism beta-dextranase and beta-glucosaccharase production method

A technology of glucosidase and compound microorganism, which is applied in the production field of compound microorganism β-glucanase and β-glucosidase, which can solve the problem of inability to degrade cellobiose and cellotriose and restrict β-glucanase Application, affecting the hydrolysis efficiency of β-glucan, etc.
CN1884506AInactive Publication Date: 2006-12-27天津科建科技发展有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
天津科建科技发展有限公司
Publication Date
2006-12-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for making composite microbio beta-glucanase and beta-glucosidase. The invention includes mixed fermentation of trichoviridin and aspergillus niger to prepare composite microbio beta-glucanase and beta-glucosidase, preparation of solid enzyme preparation and liquid enzyme preparation, and inclined-plane bacterial, shake flask shake culture, liquid seed tank enrichment culture of the trichoviridin and aspergillus niger, mixed input of the trichoviridin and aspergillus niger after enrichment culture into solid fermentation tank to perform fermentation, after fermentation, by digestion, filtration and ultrafilter membrane concentration, obtaining liquid enzyme preparation after adding protecting agent; obtaining solid enzyme preparation by adding British gum in concentrated enzymatic solution with dehumidification and granulation. The inventiom is of scientific and fair industrial design, the trichoviridin and aspergillus niger obtained by mixed culture and screening of strains of fungus can perform paragenetic mixed culture and mutually have no antagonistic reaction, the cost is low, the energy consumption is small, and the invention is of no devil liquor and waste slag discharge, which has a wide applicability of an invention and fills up our zymin breed margin, and has large market foreground.
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Description

technical field

[0001] The present invention relates to one, in particular to a preparation method of composite microbial β-glucanase and β-glucosidase. Background technique

[0002] In recent years, the research direction of β-glucan mainly focuses on the breeding of microbial production bacteria with high β-glucanase activity, and the activity of β-glucanase is used as a reflection indicator of the activity of microbial production bacteria. Ignoring the activity index of β-glucosidase leads to high activity of the produced β-glucanase, but in practical application, the hydrolysis effect is not ideal. The reason is that the β-glucosidase in the enzyme component is very low, even undetectable. However, in the absence of β-glucosidase, cellobiose and cellotriose cannot be completely degraded into glucose, which seriously affects the hydrolysis efficiency of β-glucan and restricts the application of β-glucanase. Contents of the invention

[0003] The main purpose of the pr...

Claims

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