Unlock instant, AI-driven research and patent intelligence for your innovation.

Co-expression carrier and eukaryon expressing carrier capable of inducing cell immune response

A technology of eukaryotic expression vectors and co-expression vectors, which is applied in the field of pGL3/CD80-P1A co-expression vectors and pcDNA3.1/B2M-IL15 eukaryotic expression vectors, can solve the problems of disappearance of immune stimulation and achieve the goal of overcoming immunogenic Sexually low effect

Inactive Publication Date: 2007-02-28
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The basic skeleton of the immunostimulatory sequence is 5'-purine-purine-CpG-pyrimidine-pyrimidine-3', which is mainly derived from bacterial DNA. The same synthetic sequence has a similar immune enhancement effect, but methylation modification or sequence change, then Immunostimulatory effect disappears completely

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Co-expression carrier and eukaryon expressing carrier capable of inducing cell immune response
  • Co-expression carrier and eukaryon expressing carrier capable of inducing cell immune response
  • Co-expression carrier and eukaryon expressing carrier capable of inducing cell immune response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1 Materials and methods

[0034] 1.1 Materials

[0035] Reagents: Various restriction endonucleases and T4 DNA ligase were purchased from Boehringer Mannheim Company; PCR reagents were purchased from TaKaRa Company; cell total RNA extraction reagent TRIZOL and reverse transcription reaction reagents were purchased from InVitrogen Company; DNA gel recovery kit and plasmid DNA isolation and purification kit from QIAGEN company; agarose from Promega company; yeast extract, tryptone from OXOID company; Lamda DNA Hind III marker (Huami company), 100bp DNA Marker (Tianwei Times company); single Nuclear cell separation solution LymphoprepTM (Axis-Shield polAS); MTT (Huami Corporation); ConA (purchased from Jingmei Corporation); Recombinant human IL-2 was purchased from PeproTech EC Ltd; R-PE labeled Pro5 MHC PentamerH-2L d / LPYLGWLVF, FITC-labeled rat anti-mouse CD8 monoclonal antibody (clone number: KT15) and P1A nonapeptide (LPYLGWLVF) were purchased from PROIMMUNE Company;...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates the induced cell immune response pGL3 / CD80-P1A expression carrier and pcDNA3.1 / B2M-IL15 eucaryon expression carrier. The invention has the advantages of overcoming low DNA vaccine immunity. The invention can prevent and treat tumour and virus endurance infection.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a pGL3 / CD80-P1A co-expression vector and pcDNA3.1 / B2M-IL15 eukaryotic expression vector that can induce cellular immune response. Background technique [0002] DNA vaccine, also known as nucleic acid vaccine or gene vaccine, refers to inserting an exogenous gene encoding a certain target antigen protein into a eukaryotic cell expression plasmid, directly introducing it into animal somatic cells, and passing the host cell's exogenous gene. The expression system synthesizes the antigenic protein and induces the host to produce an immune response to the antigenic protein, thereby achieving the purpose of preventing and treating diseases. Nucleic acid vaccines can not only stimulate the body to produce specific humoral immunity, but also induce specific CTL effects (cellular immunity) with cytotoxic killing function, and can directly remove target cells with target antigens. Inf...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/85C12N15/12A61K48/00C12N15/24A61P35/00A61P31/12
Inventor 张兴黔钱关祥梅文瀚方丽娟钱丽
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE