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Synthetic mammalian sulphamidase and genetic sequences encoding same

a technology of mammalian sulphamidase and genetic sequences, applied in the direction of sugar derivatives, biochemistry apparatus and processes, enzymes, etc., can solve the problems of difficult purification of target molecules, particularly in research and development, and many difficulties for practitioners

Inactive Publication Date: 2001-08-16
WOMENS & CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, practitioners have faced many difficulties in purifying target molecules to an extent sufficient to determine amino acid sequences to permit the development of oligonucleotide probes to assist in the cloning of genetic sequences encoding the target molecules.
Such difficulties have been particularly faced in the research and development of lysosomal enzymes.

Method used

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  • Synthetic mammalian sulphamidase and genetic sequences encoding same
  • Synthetic mammalian sulphamidase and genetic sequences encoding same
  • Synthetic mammalian sulphamidase and genetic sequences encoding same

Examples

Experimental program
Comparison scheme
Effect test

example 2

Characterisation of Sulphamidase

[0064] Results presented in FIG. 1 show a subunit of about M.sub.r 56,000 from sulphamidase purified from human liver, kidney and placenta The native M.sub.r is about 100,000 to 120,000.

example 3

N-Terminal Amino Acid Sequence Determination

[0065] The N-terminal amino acid sequence was determined using the methods of Clements et al (1989) and Wilson et al (1990).

[0066] The amino acid sequence is shown in Table 2.

example 4

Cloning of Sulphamidase cDNA

[0067] The N-terminal amino acid sequence (Example 3) was used to generate oligonucleotides and primers to screen a human kidney cDNA library. An approximately 2.7 kbp cDNA clone was then isolated encoding the entire sequence of human sulphamidase. The nucleotide sequence (SEQ ID NO:1) and corresponding amino acid sequence (SEQ ID NO:2) are shown in FIG. 2.

[0068] The amino acid sequence is shown in single letter code above the cDNA sequence. Nucleotide and amino acid numbers are in the right margin. The probable site of signal peptide peptidase cleavage between amino acids 20 and 21 is shown by an arrow. Amino acids colinear with the amino-terminal peptide data is underlined. The five potential N-glycosylation sites are asterisked above the peptide sequence. The open reading frame is 1506 nucleotides long and encodes a 502 amino acid protein. The predicted molecular mass of the mature protein (minus the 20 amino acid signal peptide) is about 54,679 kilo d...

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Abstract

The present invention relates generally to mammalian sulphamidase and to genetic sequences encoding same and to the use of these in the investigation, diagnosis and treatment of subjects suspected of or suffering from sulphamidase deficiency.

Description

[0001] The present invention relates generally to mammalian sulphamidase and to genetic sequences encoding same and to the use of these in the investigation, diagnosis and treatment of subjects suspected of or suffering from sulphamidase deficiency.[0002] Bibliographic details of the publications referred to by author in this specification are collected at the end of the description. Sequence Identity Numbers (SEQ ID NOs.) for the nucleotide and amino acid sequences referred to in the specification are defined following the bibliography.[0003] Throughout this specification, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or Beer or group of elements or integers but not the exclusion of any other element or integer or group of elements or integers.BACKGROUND TO THE INVENTION[0004] The increasing sophistication of recombinant DNA technology is greatly facilitati...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/00A61P3/00C12N9/14
CPCA61K38/00C12N9/14A61P3/00Y02A50/30
Inventor HOPWOOD, JOHN JOSEPHSCOTT, HAMISH STEELEFREEMAN, CRAIG GEOFFREYMORRIS, CHARLES PHILLIPBLANCH, LIANNE CHERYLGUO, XIAO HUI
Owner WOMENS & CHILDRENS HOSPITAL