Additive solution for blood preservation

a technology of additive solutions and blood, which is applied in the field of additive solutions for blood preservation, can solve the problems of periodic shortages of blood, limited liquid blood supply, and patients currently cannot collect and store with current technology

Inactive Publication Date: 2003-07-03
TRUSTEES OF BOSTON UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By way of background, currently the supplies of liquid blood are limited by storage.
There are periodic shortages of blood that occur due to donation fluctuation, emergencies and other factors.
Patients currently cannot collect and store with current technology enough autologous blood for certain pre-planned surgeries, including hip replacement, organ transplantation and the like.
Storage of frozen blood is known in the art but such frozen blood has limitations.
However, frozen blood is difficult to handle.
It must be thawed which makes it impractical for emergency situations.

Method used

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  • Additive solution for blood preservation
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Examples

Experimental program
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Effect test

example 2

OFAS3: Effect of pH and Oxygen Depletion on % of Cells Exposing Phosphatidylserine

[0034] Results of experimentation to determine the effect of pH and oxygen depletion on the % of red blood cells exposing phosphotidylserine with samples containing oxygen-free additive solution (OFAS3) are presented in FIG. 2. Data were obtained by flow cytometer measurements using FITC-Annexin IV probe. Each point on the graph is the average of 6 subjects. There is a significant reduction in exposed phosophatidylseine after 10 weeks when pH 8.3 and pH 6.5 blood samples, both oxygen depleted, are compared.

example 3

OFAS3: Effect of pH and Oxygen Depletion on Red Blood Cell Hemolysis

[0035] Results of experimentation to determine the effect of pH and oxygen depletion on red blood cell hemolysis with blood samples containing oxygen-free additive solution (OFAS3) are presented in FIG. 3. Each point on the graph is the average of 6 subjects. Three different pH's were tested, pH 6.5, pH 7.4, and pH 8.3, with control cultures that were not oxygen-depleted. At week 16, the pH 6.5 oxygen-depleted refrigerated red blood cell storage system has the lowest extent of hemolysis.

example 4

Addition of Metabolic Supplements During Refrigerated, Oxygen-Depleted Red Blood Cell Storage: Effect of Metabolic Supplements Added at Different pH's in the Presence or Absence of Oxygen on Cellular ATP Levels

[0036] Results of experimentation to determine the effect of addition of metabolic supplements added during refrigerated, oxygen-depleted storage of red blood cells at different pH's in the presence or absence of oxygen on cellular ATP levels, are graphically presented in FIG. 4. Two different pH's were tested, pH 6.5 and pH 8.3, with control cultures that are not oxygen depleted. Metabolic supplement, Rejuvesol, was added to cultures as indicated by the arrows in FIG. 4, which correspond approximately to additions during cold storage at 9, 14, and 21 weeks respectively. These data show that ATP levels are significantly increased each time the cold fuel / metabolic supplement is added. The highest ATP levels are sustained with pH 6.5 additive solution under oxygen depleted condi...

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Abstract

There is provided compositions and methods for the storage of red blood cells. The compositions are additive solutions comprising adenine, dextrose, mannitol, NaH2PO4, and optionally NaCl and / or NH4Cl. Composition are preferably used with oxygen-depletion refrigerated storage of red blood cells and may optionally be employed with nutrient supplements extending the useful shelf life of stored blood.

Description

[0001] This application claims priority from U.S. Provisional Application No. 60 / 322,405 filed Nov. 16, 2001.[0003] The present invention relates to the preservation of blood in liquid form. More particularly, the present invention relates to compositions and methods useful for the storage of red blood cells. Still more particularly, compositions and methods useful for refrigerated storage of red blood cells in oxygen-depleted refrigeration are provided.DESCRIPTION OF THE PRIOR ART[0004] By way of background, currently the supplies of liquid blood are limited by storage. Stored blood expires after about 42 days of refrigerated storage. Red blood cells may, for example, be stored under refrigeration at a temperature above freezing (4.degree. C.) as packed blood cell preparations. Red blood cells may be concentrated from whole blood with separation of the liquid blood component (plasma). Expired blood cannot be used and is discarded. There are periodic shortages of blood that occur du...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0226C12N5/0641
Inventor BITENSKY, MARK W.YOSHIDA, TATSURO
Owner TRUSTEES OF BOSTON UNIV
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