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Method and Kits for Repairing Nucleic Acid Sequences

a nucleic acid sequence and repair kit technology, applied in the field of forensic analysis, can solve the problems of unable to be used as evidence in criminal prosecutions, unable to effectively analyze dna samples, and unable to perform dna analysis in forensic laboratories

Inactive Publication Date: 2010-01-14
GENERAL ELECTRIC CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DNA analysis performed at forensic laboratories may be hampered by DNA samples that have been exposed to uncontrolled environmental conditions.
In the event of such damage, the DNA sample often cannot be analyzed effectively and thus cannot be used as evidence in criminal prosecutions.

Method used

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  • Method and Kits for Repairing Nucleic Acid Sequences
  • Method and Kits for Repairing Nucleic Acid Sequences
  • Method and Kits for Repairing Nucleic Acid Sequences

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Embodiment Construction

[0018]Damaged DNA may contain nucleotide modifications and / or breaks (single- or double-stranded) in the DNA that are a block to the standard DNA replication machinery of the cell. The pathways to the repair of damaged nucleic acids may be broken down into four categories: ligation to seal nicks; direct reversal of a nucleotide modification to normal state; recombinational repair using a second DNA copy as template; and excision repair. The methods described herein may employ enzymes to perform one or more of these repair techniques to repair damaged DNA. Methods for DNA repair described herein may be used, for example, for detection of pathogenic organisms, forensic purposes, medical diagnostic purposes, or clinical purposes. Embodiments are also provided that relate generally to methods applicable in analytical, diagnostic, or prognostic applications.

[0019]FIG. 1 illustrates an exemplary process 10 by which the methods described herein for DNA repair may be effectively used in ana...

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Abstract

Methods and kits for DNA repair are provided. The methods and kits described herein repair multiple types of DNA damage. The kit may include a plurality of enzymes to repair a greater variety of lesions than any single enzyme is capable of repairing. Repair of damaged DNA may include releasing damaged bases from the DNA strand, nicking the DNA at the damaged sites, translating the nicks via 5′-3′ exonuclease activity, and sealing the nicks. The enzymes employed in the repair process may then be heat-inactivated, thereby obviating a purification process. The repaired DNA may then be analyzed using a variety of DNA analysis methods.

Description

REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY[0001]The present application contains a Sequence Listing of SEQ ID NOS 1-28 in file “197952-1_sequence_listing—07JUL2008.txt” (5.1 kilobytes), created on Jun. 13, 2008, concurrently submitted with the specification by electronic filing, which is herein incorporated by reference in its entirety.BACKGROUND[0002]The invention relates generally to forensic analysis, and, more specifically, to repair of damaged nucleic acid sequences.[0003]In criminal investigations, investigators often look for physical evidence left behind at the scene of a crime to link a suspect to the crime. For example, physical evidence has traditionally included fingerprints, hairs, fibers, and so forth. A modern forensic analysis of physical evidence may include analysis of evidence, such as blood or other bodily fluids, which contains deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) specimens. Forensic laboratories analyze DNA and / or RNA samples in an ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12P19/30C12N9/24
CPCC12N9/93C12N9/1252
Inventor NELSON, JOHN RICHARDGAO, WEI
Owner GENERAL ELECTRIC CO
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