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Apparatus and Method for Segmented Thermal Cycler

Inactive Publication Date: 2010-05-20
LIFE TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007]According to various embodiments, the present teachings describe a thermal cycler for processing biological or chemical samples with a plurality of reaction vessel receiving elements configured to receive one standard microtiter plate, a plurality of thermoelectric cooling devices (TEC) disposed to correspond to each of the plurality of reaction vessel receiving elements, wherein the TEC provides heating and cooling, a drip pan positioned above the TECs and framing the plurality of reaction vessel receiving elements, a single gasket to seal the plurality of reaction vessel receiving elements, wherein the gasket has a convex construction

Problems solved by technology

At a higher elongation temperature, the addition of the bases is accelerated, with the probability of errors increasing with higher temperature.
Problems arise with conventional devices as these parameters can not be varied in one test series for an individual reaction vessel holder.

Method used

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  • Apparatus and Method for Segmented Thermal Cycler
  • Apparatus and Method for Segmented Thermal Cycler
  • Apparatus and Method for Segmented Thermal Cycler

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Embodiment Construction

[0022]In the following description, reference is made to the accompanying drawings that form a part thereof, and in which are shown by way of illustration specific exemplary embodiments in which the invention may be practiced. These embodiments are described in sufficient detail to enable those skilled in the art to practice the invention and it is to be understood that other embodiments may be utilized and that changes may be made without departing from the scope of the invention. The following description is, therefore, not to be taken in a limited sense.

[0023]As used herein, the term “microtiter plate” is also known as a “sample plate,”“microtitration plate,” and “microplate” interchangeably and refers to a multi-welled sample receptacle for testing of chemical and biological samples. Microplates can have wells that are conical, cylindrical, rectilinear, tapered, and / or flat-bottomed in shape, and can be constructed of a single material or multiple materials. A standard microtite...

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Abstract

The present invention relates to a thermal cycler for the carrying out of chemical or biological reactions, such as PCR or other nucleic acid amplification reactions, that is segmented with a plurality of reaction vessel receiving elements. The reaction vessel receiving elements are thermally isolated from each other and provide an airtight seal to prevent liquids or moisture from penetrating below the reaction vessel receiving elements. The reaction vessel receiving elements have several recesses arranged in a pattern to receive the reaction vessels of a single standard microtiter plate and the segmented thermal cycler has a system for independently heating and cooling each of the reaction vessel receiving elements.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This applications claims a priority under 35 U.S.C. §119(e) from U.S. Provisional Patent Application No. 61 / 114,902 filed Nov. 14, 2008, which is incorporated herein by reference.FIELD OF THE INVENTION[0002]The present invention relates to a thermal cycler for the carrying out of chemical or biological reactions, such as PCR or other nucleic acid amplification reactions, the thermal cycler is segmented in that it has a plurality of reaction vessel receiving elements separated and thermally isolated from each other for receiving reaction vessels, wherein the reaction vessel receiving elements have several recesses arranged in a pattern to receive the reaction vessels of a single standard microtiter plate. The segmented thermal cycler has a system for independently heating and cooling each of the reaction vessel receiving elements.INTRODUCTION[0003]Testing of biological or chemical samples often requires a device for repeatedly subjecting mu...

Claims

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Application Information

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IPC IPC(8): C12P19/34B01L9/00B01L7/00C12M1/34
CPCB01L7/52B01L9/523B01L2300/1822B01L2300/0654B01L2300/0829B01L2200/0689B01L2300/041B01L2300/0663B01L2300/1833B01L2300/1894C12Q1/686
Inventor NG, HWEE TIANYEO, HUEICHOO, WEI HSIEN
Owner LIFE TECH CORP
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