Skin equivalent culture

a technology of skin and composition, applied in the field of skin equivalent culture, can solve the problems of concave rather than concentric shape, loss of height of the construct, and doubt that the collagen will obtain its final structur

Inactive Publication Date: 2011-02-03
SMITH & NEPHEW INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0033]In one aspect, the connective tissue equivalent is formed using two or more stacked freeze-dried constructs. At least two of the two or more freeze-dried constructs may

Problems solved by technology

This results in a concave rather than the desired convex shape, and loss in height of the construct.
Whilst it is accepted that collagen will be produced in the period of incubation (10 days) as described by Hewitt et al., without the addition of substantial means of synthesizing or obtaining ascorbic acid (vitamin C) it is doubt

Method used

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Embodiment Construction

Discussion of Prior Art and the Present Invention

[0048]D1—Hansbrough J F, Morgan J L, Greenleaf G E and Bartel R. (1993). Composite grafts of Human keratinocytes grown on a polyglactin mesh-cultured fibroblast dermal substitute function as a bilayer skin replacement in full-thickness wounds on athymic mice. J. Burn Care and Rehab. 14 (5), 485-494.[0049]D2—Geesin et al, Regulation of Collagen Synthesis in human dermal fibroblasts in contracted collagen gels by ascorbic acid, growth factors, and inhibitors of lipid peroxidation. Experimental Cell Research 206, 283-290.[0050]D3—WO 03 / 41568 (University of New Jersey, Hewitt et. al) A Three dimensional matrix for producing living tissue equivalents.

Overview of ICX-SKN—Skin Equivalent Culture, According to the Invention:

[0051]ICX-SKN has been designed as an active cell therapy consisting of human dermal fibroblasts (HDFs) embedded in fibrin gels which are allowed to mature over several weeks. A key aspect of this maturation period is that...

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Abstract

Disclosed is a method of preparing a collagenous construct comprising casting a support matrix comprising fibrin and viable collagen-producing cells onto a support material, wherein said cells include human dermal fibroblasts, incubating in situ said support matrix in a collagen-inducing medium thereby, inducing or enhancing collagen production by said cells to form a collagenous construct, and degrading said fibrin, and rendering said collagenous construct free of said viable collagen-producing cells.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of U.S. application Ser. No. 11 / 886,113, filed Sep. 11, 2007, which is national phase application under 35 U.S.C. §371 of International Application No. PCT / GB2006 / 000890, filed Mar. 14, 2006, which claims the benefit of Great Britain Application No. 0505202.2, filed Mar. 14, 2005. The contents of all of these referenced applications are incorporated by reference.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The present invention relates to methods of forming soft connective tissue compositions such as skin equivalents, compositions made by the methods and their uses.[0004]2. Description of Related Art[0005]Bioengineered skin substitutes have emerged over the past two decades, with the initial intention to replace autograft, allograft, and xenograft in burn applications. Examples of engineered skin substitute products available or anticipated in the market are shown in Table 1. Several o...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K38/39A61P17/02C12P21/02A61L27/38A61L27/60C12N5/071
CPCA61L27/3804A61L27/3886A61L27/3895A61L27/60C12N2533/56C12N2501/11C12N2501/15C12N2502/094C12N2502/1323C12N5/0698A61P17/02A61L27/20A61L27/225A61L27/24A61L27/3687A61L27/3691A61L27/3839A61L27/54C12N2500/25C12N2500/30C12N2500/32
Inventor KEMP, PAULSHERING, DAVIDSHERING, ANDREWJOHNSON, PENNYMARSHALL, DAMIAN
Owner SMITH & NEPHEW INC
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