Methods for enhanced root nodulation in legumes
a legume and root nodulation technology, applied in the field of legume root nodulation enhancement, can solve the problems of reducing the number of rhizobial populations, increasing the cost of processing requirements, and limiting their availability, so as to increase the protein content of plants, increase the yield of plants, and enhance the root nodulation
Inactive Publication Date: 2012-07-05
ADVANCED BIOCATALYTICS
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Benefits of technology
[0012]Disclosed herein are methods of increasing, enhancing, or accelerating root nodulation in a plant, accelerating growth of nitrogen fixing bacteria in nodules of a plant, increasing protein content in a plant, increasing yield of a plant, improving water retention of a plant, or reducing water use of a plant, the method comprising identifying a plant in need of root nodulation, and applying to the plant a composition comprising a protein component comprising yeast stress proteins resulting from subjecting a mixture obtained from the yeast fermentation to stress.
Problems solved by technology
The handling requirements increase costs and further limit their availability through standard distribution.
A key limitation with inoculating legumes to maximize yield is that even under the best storage conditions, rhizobial populations will decline over time.
Tan colored nodules are not actively fixing bacteria and white, grey or green colored nodules are doing little nitrogen fixing or could be dying.
Adding nitrogen fertilizer can be detrimental because some legumes don't respond to nitrogen fertilizer.
In other cases, because the nitrogen fixation process is energy intensive the plant may not expend energy for nodulation if it can absorb nitrogen directly from the added fertilizer using less energy.
This process uptakes less nitrogen than by fixation and yields can be compromised.
But the higher rate of nodule formation does not always translate into higher yield.
Typically, only a small amount might leak to neighboring plants.
U.S. Pat. No. 6,855,536 states that, “Unfortunately, for most of the United States, inoculation has been shown to be ineffective.
Method used
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example 1
[0055]A fermentation mixture derived from the fermentation of Saccharomyces cerevisieae in which the yeast cells are stressed by raising the temperature to at least 35° C. for at least two hours, then cooling to <30° C. centrifugation. Upon removal of the yeast cells by centrifugation the pH is adjusted to 4.0 and sodium benzoate and 21.1% propylene glycol is incorporated to provide stability.
PSCLinear Primary Alcohol (C12-C15), 7 mole Ethoxylate7.5%Sodium Lauryl Ether (3 mole) Sulfate (60%)2.5%Stabilized Fermentation Mixture 23%Water 67%TOTAL100%
[0056]Host plants: Peas, Pisum sativum. Two seeds were sown in 6 inch diam pots (approx. 3500 ml volume) filled with UC Mix II (Matkin and Chandler 1957). Soil Mix II is formulated with plaster sand, bark, peat moss, Dolomite, limestone flour, triple super phosphate, potassium nitrate, muriate of potash, ferrous sulfate, copper sulfate, magnesium sulfate, zinc sulfate, and manganese sulfate. Once sown, the pots were watered on a daily basi...
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Disclosed herein are methods of increasing, enhancing, or accelerating root nodulation in a plant, accelerating growth of nitrogen fixing bacteria in nodules of a plant, increasing protein content in a plant, increasing yield of a plant, improving water retention of a plant, or reducing water use of a plant, the method comprising identifying a plant in need of root nodulation, and applying to the plant a composition comprising a protein component comprising yeast stress proteins resulting from subjecting a mixture obtained from the yeast fermentation to stress.
Description
RELATED APPLICATION[0001]The present application claims priority to the U.S. Provisional Application Ser. No. 61 / 399,095, filed Jul. 7, 2010, and entitled “Methods for Enhanced Root Nodulation in Legumes,” the entire disclosure of which is incorporated by reference herein.FIELD OF THE INVENTION[0002]The present invention relates to enhancement of plant growth and crop yield by applying compositions of fermentation liquids and surfactant that improve root nodulation by rhizobacteria, e.g., Rhizobium, Bradyrhizobium, Sinorhizobium, etc.BACKGROUND OF THE DISCLOSURE[0003]Legumes are plants, such as alfalfa, clover, peas, beans, lentils, lupins, mesquite, carob, soy, peanuts, locust trees (Gleditsia or Robinia), wisteria, and the Kentucky coffeetree (Gymnocladus dioicus), that form a symbiotic relationship between their roots and bacteria, specifically of the family Rhizobiaceae. The bacteria penetrate the plant root hairs, and then induce the formation of nodules. The plant provides the...
Claims
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Login to View More IPC IPC(8): A01N63/02A01P21/00A01N63/50
CPCA01N63/04Y02A40/10A01N63/50A01N63/32A01N25/30A01P21/00
InventorPODELLA, CARL W.BALDRIDGE, JOHN W.MICHALOW, ANDREW H.GOLDFELD, MICHAEL G.
OwnerADVANCED BIOCATALYTICS