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Chromatography column support

a technology for chromatography columns and supports, applied in the direction of machine supports, instruments, separation processes, etc., can solve the problem of complex design of large-scale preparative chromatographic processes

Inactive Publication Date: 2013-11-21
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text describes the construction of a chromatography column support that minimizes disturbances to the chromatography material during packing and the mobile phase flow during chromatographic separation. The use of a connector with circular legs and perforated plates is reported as a stabilizing solution for large diameter chromatography columns. The technical effect of the patent text is to improve the stability and efficiency of chromatographic separations.

Problems solved by technology

The design of large-scale preparative chromatographic processes can be very complex and can involve many factors that are not primarily “chromatographic”.

Method used

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  • Chromatography column support
  • Chromatography column support
  • Chromatography column support

Examples

Experimental program
Comparison scheme
Effect test

example 1

Packing of a Chromatography Column Comprising a Support as Reported Herein

[0213]During the packing process of columns which are equipped with the flexible bed support elements as reported herein the homogeneous inserting and distribution of the chromatography medium is very important. There are two possibilities to reach a homogeneous packing. The first is to put the flexible bed support element in the column first and inserting the slurry afterwards. In this case the slurry must homogeneously be brought in into the column. The other possibility is bringing in the slurry into the column first, homogenizing it in the column and afterwards introducing the bed support element by putting it directly into the slurry in the column. During the settling a homogeneous column bed forms and encloses the element. After the specified settling time can either an intermediate column fit be put on the bed support element or the column can be locked with an adapter. If an intermediate column frit is...

example 2

Chromatographic Separation Using a Conventional Chromatography Column not Comprising a Support as Reported Herein—Cycle Number and Pressure Characteristics for the use of the Chromatography Material

[0214]Herein the life cycle of a conventionally packed HPLC column is shown. It can be seen from FIG. 7 that the pressure directly after packing is about 40 bars. This increases after the fortieth regeneration cycle to about 70 bars.

[0215]Packing details:[0216]Column diameter: 30 cm[0217]Column type: dynamic compression without bed support elements and intermediate column frits[0218]Bed height: about 40 cm[0219]Used chromatographic material: Vydac C4[0220]Flow rate: 162 l / h

[0221]Pressure values during the regenerations and cycle numbers are shown in FIG. 7.

example 3

Chromatographic Separation Using a Conventional Chromatography Column Comprising a Support as Reported Herein—Cycle Number and Pressure Characteristics for the use of the Chromatography Material

[0222]Herein is shown a HPLC column with a support as reported herein and an intermediate column frit. It can be seen that a lower initial pressure after packing compared to the column of Example 2 can be obtained. Altogether a more stable pressure course can be obtained over the use cycles. Also a maximum cycle number of 98 can be reached without the need to repack the column. The intermediate column frit without the support as reported herein fulfills a bed support function at the HPLC media which are compressible only in a very low measure.

[0223]Packing details:[0224]Column diameter: 15 cm[0225]Column type: dynamic compression with intermediate column frit[0226]Bed height: about 40 cm[0227]Used chromatographic material: Vydac C4[0228]Flow rate: 35 l / h

[0229]Pressure values during the regene...

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PUM

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Abstract

Herein is reported the use of a chromatography column support comprising at least one plane of symmetry, one axis of symmetry, at least three legs, at least three straight connectors, whereby the connectors define a plane that is perpendicular to the axis of symmetry of the support, whereby the connectors are connected to each other at the axis of symmetry, whereby each leg is connected to a connector, whereby each leg is perpendicular to the plane defined by the connectors, whereby all legs are on the same side of the plane defined by the connectors for stabilizing the packing of a chromatography column.

Description

[0001]Herein is reported a chromatography column support which allows a movable frit to be positioned within a chromatography column at a desired position. The support comprises a multitude of legs and a multitude of connectors connected to the upper end of the legs providing a support over the entire diameter of the column for a movable frit.BACKGROUND OF THE INVENTION[0002]The design of large-scale preparative chromatographic processes can be very complex and can involve many factors that are not primarily “chromatographic”. These factors include method of column packing, hydrodynamic pressure drop, and flow distribution. These ultimately factor into the economics of the process.[0003]Stickel, J. J. and Fotopoulos, A. (Biotechnol. Prog. 17 (2001) 755-751) report pressure-flow relationships for packed beds of compressible chromatography media at laboratory and production scale.[0004]For gel filtration columns at technical scale the problem of compaction of the gel filling resulting...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): B01D15/22
CPCB01D15/22G01N30/56G01N30/6021G01N30/603G01N30/6047G01N30/6069G01N2030/565G01N30/60
Inventor BLASCHYK, ANDREAS
Owner F HOFFMANN LA ROCHE & CO AG
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