Delivery of viral agents

a technology of viral agents and delivery methods, applied in the direction of antibacterial agents, drug compositions, biocides, etc., can solve the problems of diluted effectiveness, high health care costs, and often failed antimicrobial chemicals application to prevent infection from becoming established or recurring, so as to improve the growth rate of potato plants and reduce the incidence of infection , the effect of reducing the incidence of infection

Active Publication Date: 2014-07-24
FIXED PHAGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0069]In a further specific embodiment of the invention, potato tubers were treated with immobilised phage. In detail, phage was attached to the surface of cellulose particles and these were immobilised onto the potato surface by spraying the tubers with a solution of the particles and then drying the tubers—to enable the particles to adhere. Growth of the potato plants was surprisingly improved followed this treatment: incidence of infection was reduced, and also growth of the treated plants was significantly better.
[0070]In a

Problems solved by technology

Infection can transform relatively minor lesions into chronic wounds that increasingly pose major health care problems, particularly for the elderly, diabetic or infirm.
Unfortunately even the application of strong antimicrobial chemicals is frequently unsuccessful in preventing infection becoming established or recurring.
In large part this may be due to the inability of the disinfecting agent to completely eradicate all problem bacteria.
Additionally, as is well known, many bacteria are now resistant to antibiotics and their effectiveness becomes diluted over time.
Central problems associated with application of bacteriophages as a means of counteracting infection have been deriving an appropriate means of delivery whilst ensuring bacteriophages remain stable and viable long enough to exert their powerful antimicrobial effects.
Administration in liquid

Method used

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  • Delivery of viral agents
  • Delivery of viral agents
  • Delivery of viral agents

Examples

Experimental program
Comparison scheme
Effect test

example 1

Effectiveness of LVCC for Delivery of Bacteriophage to Counteract Deep Wound Infection

[0106]FIG. 1 shows schematically an LVCC of the invention (1) pressed onto a wound (4) in a tissue (3) by dressing (2).

[0107]FIG. 2a is an inverted dressing of the invention incorporating an LVCC, shown in cross section. A pouch (5) encloses an LVCC (6) adhered to dressing (7), having top dressing surface (8)—hence it is inverted in the figure.

[0108]FIG. 2b shows schematically an LVCC (9) woven into a dressing (10). The two filaments (9a and 9b) are of different colours and have different bacteriophage attached. FIG. 3 shows a further schematic of a dressing (11) into which a filament with phage attached (12) is incorporated.

[0109]In a first example the effectiveness of an LVCC for delivering bacteriophage, to counteract bacterial infection within an infected deep wound environment using an in vivo model is demonstrated. Deep wounds penetrating several tissue layers and infected at all levels with ...

example 2

Extended Stability

[0113]LVCC's recovered from infected subjects were washed and stored at 4° C. and periodically evaluated for continuing antimicrobial activity against MRSA grown in vitro. In a series of experiments anti-MRSA activity was maintained as demonstrated by LVCC placed within a lawn of bacteria. Bacteriophage activity (as shown by the clearing seen around the recovered LVCCs) extended several weeks following storage—see FIG. 5.

example 3

Stability Conferred by LVCC Against Desiccation, Ultra Violet Radiation and Temperature

[0114]In these examples LVCC were generated using activated nylon polymer or cotton fibres as the carrier substrate. LVCC with attached bacteriophage were exposed to a series of environmental conditions, including dehydration, shown by the effect of relative humidity on bacteriophage survival (see FIG. 6a), ultraviolet light (see FIG. 6b) and elevated temperature (see FIG. 6c), all conditions known to denature free bacteriophages.

[0115]Three 2×1 cm phage strips were placed on LBM agar and exposed to UV light in the sterile cabinet for 5, 15 and 30 min. The strips were then turned over and placed on a fresh area of agar and the UV exposure repeated. Control phage strips were treated similarly, but were shielded from UV. The strips were then transferred to 15 ml LB broth, inoculated with 50 ul Staphylococcus aureus suspension and incubated.

Results—see Table 2

[0116]Conclusion: Attached phage can with...

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PUM

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Abstract

Plant material has bacteriophage has been attached, wherein the bacteriophage retains infectivity. The plant material includes fruits, vegetables, leaves, stems, flowers, roots, tubers, seedlings and seeds. Plant diseases and animal diseases can be treated. A separate composition comprises a carrier selected from (i) a filament, (ii) a planar material, and (iii) particles and/or beads, and bacteriophage covalently attached thereto, wherein the bacteriophage retains infectivity, useful in treatment or prevention of bacterial infection in a deep wound.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a means for the delivery of viral agents (such as bacteriophage or other viruses), in a sustainable viable state, and in various formats suitable for prevention and control of microbial infection, colonisation and / or contamination. A system is provided which may also act as a means to locate and deliver genetically modified viable viruses suitable for delivery of specific genes / proteins as therapy or immunisation. The invention can be applied to counteract microbial / bacterial growth and / or colonisation in virtually any situation in which microbial / bacterial contamination, colonisation or infection is present or likely to occur and where the sustained antimicrobial activity of viruses such as bacteriophages might be beneficial.BACKGROUND TO THE INVENTION[0002]It is now generally accepted that microbial / bacterial contamination or infection is a major factor influencing normal biological / physiological functions (such as heali...

Claims

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Application Information

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IPC IPC(8): A61K47/48A01N63/00A61K35/76A01N63/40A61K35/768
CPCA61K47/48776A01N63/00A61K35/76A61L15/36A61L15/44A61L2300/404A61L2300/442A61K35/768C12N2795/00032A01N37/46A23K10/10A23K10/18A23K10/30A23K50/70A23K50/75A61P1/00A61P31/04A01N63/40
Inventor CHADWICK, JAMESMATTEY, MICHAEL
Owner FIXED PHAGE
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