Method for treating inflammatory conditions
a technology for inflammatory conditions and treatment methods, applied in the field of treatment methods for inflammatory conditions, can solve the problems of osteoporosis, peptic ulcers, osteoporosis,
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example 1
Development of Rheumatoid Arthritis in a Prophylactic Model of Rat Adjuvant Induced Arthritis (AIA)
[0121]Lewis rats (Harlan, R #2359) weighing 130-150 grams were received and housed in cages with 3 rats per cage. The animals were placed in quarantine and monitored daily for signs of disease or clinical distress. The rats were marked with ear tags to identify animal number. Following the quarantine period, the animals were moved to a different room for routine maintenance and housing. 75 mg of Mycobacterium tuberculosis H37 RA (Difco Cat#231141) was homogenized in 10 ml squalene (Sigma Cat# S-3626) for use as adjuvant. Prior to injection of the adjuvant, the rats were weighed, paw volumes measured, and shaved at the dorsal base of the tail. The rats that received the adjuvant (all rats excluding the non-disease group) were injected with 100 μl of adjuvant intradermally at the base of the tail. The non-disease rats received phosphate buffered saline instead of adjuvant.
[0122]Rat weigh...
example 2
Evaluation of TC5 at 10 mg / Kg / Day Dosing in a Male Rat AIA Prophylactic Model
[0123]Male rats were subjected to arthritis induction as described in Example 1. In this prophylactic model of AIA, TC5 at 10 mg / kg / day dosing was injected IP in male rats. The paw volumes were measured every day using plethysmometer. The readouts for each rat were plotted against the study day. ENBREL at 30 mg / kg dose was injected only once a week subcutaneously. The animals were dosed for 14 days following which they were euthanized for other readouts.
[0124]As shown in FIGS. 1-2, TC 5 reduced paw inflammation to control levels similar to those observed for ENBREL. ENBREL was used as a positive control. Animals were also weighed at each time points. Weight loss was found to be near negligible compared to control ENBREL group as shown in FIG. 3. The large error bars reflect smaller group size.
[0125]Radiographs shown in FIG. 4 demonstrate that TC5 was effective is preserving the joint damage. FIG. 5 shows co...
example 3
Evaluation of TC5 at 2.5 mg / Kg / Day and 500 ug / Kg / Day Dosing in a Male Rat AIA Prophylactic Model
[0126]Male rats were subjected to arthritis induction as described in Example 1. In this prophylactic model of AIA, TC5 at 2.5 mg / kg / day dosing or 500 μg / kg / day was injected IP in male rats. The paw volumes were measured every day using plethysmometer. The readouts for each rat were plotted against the study day. ENBREL at 30 mg / kg dose was injected only once a week subcutaneously as a positive control. The animals were dosed for 14 days following which they were euthanized for other readouts.
[0127]As shown in FIGS. 7-8, TC 5 at 2.5 mg / kg demonstrated maximal effect similar to ENBREL. A lower dose of 500 μg / kg / day also reduced paw inflammation. ENBREL was used as a positive control. Animals were weighed at each time point. Weight loss was found to be near negligible compared to disease group as shown in FIG. 9. The large error bars reflect smaller group size.
[0128]Radiographs shown in FIG...
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