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Method of manufacturing protein array or peptide array, method of identifying functional protein or functional peptide, protein array or peptide array, and functional protein or functional peptide identification kit

Inactive Publication Date: 2016-10-06
THE UNIV OF TOKYO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention allows for efficient screening of functional proteins or peptides.

Problems solved by technology

Further, the protein array proposed in Patented Literature 2 is difficult to recover DNA after the screening of a functional protein, and has room for improvement in the efficient screening of the functional protein.

Method used

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  • Method of manufacturing protein array or peptide array, method of identifying functional protein or functional peptide, protein array or peptide array, and functional protein or functional peptide identification kit
  • Method of manufacturing protein array or peptide array, method of identifying functional protein or functional peptide, protein array or peptide array, and functional protein or functional peptide identification kit
  • Method of manufacturing protein array or peptide array, method of identifying functional protein or functional peptide, protein array or peptide array, and functional protein or functional peptide identification kit

Examples

Experimental program
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first embodiment

[0122]The protein array or peptide array according to the present embodiment comprises: a reactor array comprising a reactor having a specific aperture shape; and a nucleic acid immobilized on a solid support disposed in the reactor and a protein or peptide encoded by the nucleic acid.

[0123]The reactor comprises a protein capture molecule or peptide capture molecule for capturing the protein or peptide, the capture molecule being immobilized on at least a portion of the wall surface and bottom surface in the reactor.

[0124]As described in the first embodiment of the >, the protein array or peptide array of the present embodiment is a protein array or peptide array in which a nucleic acid is immobilized on a solid support. The nucleic acid is preferably a nucleic acid immobilized on a magnetic bead (refer to FIG. 1).

second embodiment

[0125]The protein array or peptide array according to the present embodiment comprises: a reactor array comprising a reactor having a specific aperture shape; and DNA immobilized on a solid support disposed in the reactor and a protein or peptide encoded by the DNA.

[0126]The reactor comprises an mRNA synthesized from the DNA immobilized on at least a portion of wall surface and bottom surface in the reactor, and a nucleic acid linker having a protein linking moiety or a peptide linking moiety capturing the protein or peptide.

[0127]As described in the second embodiment of the >, in the protein array or peptide array of the present embodiment, the nucleic acid linker having a protein linking moiety or a peptide linking moiety is used instead of the protein capture molecule or peptide capture molecule that has been described in the first embodiment (refer to FIG. 2).

third embodiment

[0128]The protein array or peptide array according to the present embodiment comprises: a reactor array comprising a reactor having a specific aperture shape; and DNA disposed in the reactor and a protein or peptide encoded by the DNA.

[0129]The reactor comprises an mRNA synthesized from the DNA immobilized on at least a portion of wall surface and bottom surface in the reactor, and a nucleic acid linker having a protein linking moiety or a peptide linking moiety capturing the protein or peptide.

[0130]As described in the third embodiment of the >, in the protein array or peptide array of the present embodiment, the DNA not immobilized on the solid support is used instead of the DNA immobilized on the solid support, which has been described in the second embodiment (refer to FIG. 3).

[0131]>

[0132]The functional protein or functional peptide identification kit according to the present embodiment comprises: the aforementioned > of the first embodiment; and a substrate having an affinity ...

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Abstract

There is provided a method of manufacturing a protein array or peptide array suitable for an efficient screening of a functional protein or functional peptide. The method of manufacturing a protein array or peptide array includes the steps of: (a) preparing a nucleic acid immobilized on a solid support and a cell-free synthesis system in a reactor, in which a reactor array includes the reactor having a specific aperture shape and a protein capture molecule or a peptide capture molecule provided on at least a portion of wall surface and bottom surface in the reactor; and (c) synthesizing a protein or peptide from the nucleic acid using the cell-free synthesis system and immobilizing the protein or peptide in the reactor.

Description

TECHNICAL FIELD[0001]The present invention relates to a method of manufacturing a protein array or peptide array, a method of identifying a functional protein or functional peptide, a protein array or peptide array, and a functional protein or functional peptide identification kit.DESCRIPTION OF THE RELATED ART[0002]New functional proteins are expected to contribute to biological applications, such as pharmaceuticals, detergents, food processing, reagents for research and development, clinical analysis, bioenergy, and biosensors.[0003]Regarding the acquisition of new functional proteins, protein engineering techniques to design a protein from the structural information of the protein by human intelligence were mainstream techniques. However, in order to obtain a more useful function protein, it is necessary to efficiently screen proteins than techniques known in the related art, and evolutionary molecular engineering techniques repeating the random molecular structure modification a...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1075C07K17/14C07K1/04C40B30/04G01N33/6842G01N33/6845
Inventor ICHIKI, TAKANORIUENO, SHINGOOSAWA, HISAO
Owner THE UNIV OF TOKYO
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