Method for suppressing cell growth

a cell growth and cell technology, applied in the field of cell growth suppression, can solve the problem that codons are not necessarily uniformly employed in the target cell, and achieve the effects of suppressing cell growth, high efficiency, and suppressing viral protein synthesis

Inactive Publication Date: 2017-03-16
JAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach allows for the suppression of target cell growth and virus resistance without limitations on target cell or protein types, effectively preventing viral infection and spreading across various species, including humans, by monopolizing tRNA corresponding to low-frequency codons.

Problems solved by technology

Codons are not necessarily uniformly employed in a target cell.

Method used

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  • Method for suppressing cell growth
  • Method for suppressing cell growth
  • Method for suppressing cell growth

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embodiment 1

1. Experimental Materials and Methods

(1) Bacterial Strains, Phages, and Drugs Employed, and Plasmid Construction

[0138]Artificial GFP genes 1gfp, and hgfp were synthesized by designing gene sequences based on a codon use frequency database (Nakamura, Y., Gojobori, T. & Ikemura, T., (2000), Nucleic Acids Res. 28, 292) on the basis of the amino acid sequence of the gfpmut3 gene, and relying on GenScript (Piscataway N.J., USA). The DNA sequences of 1gfp and hgfp are recorded in the DDBJ database under Registration Nos. AB304879 and AB30487. Deletion mutant genes 1gfpΔ1, 1gfpΔ2, and 1gfpΔ3 had 75%, 50%, and 25% of the length from the N-terminus of the 1gfp gene, respectively, and were obtained by PCR amplification using the 1gfp gene as template. The CAI value of the artificial deletion mutant genes were calculated using the site with the following URL: http: / / www.evolvingcode.net / codon / cai / cai.php#146. All of the plasmids employed in the bacterial experiments were constructed (14, 29, 4...

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Abstract

Disclosed is a method for suppressing the growth of a target cell, which is not limited in the type of a target cell and the type of a protein to be expressed in the target cell and needs not any preparatory experiment for determining a codon to be contained in a protein to be expressed in a target cell. Specifically disclosed is a method for suppressing the growth of a target cell, which comprises the steps of: incorporating DNA containing a region encoding a protein into the target cell, and allowing a protein encoded by the DNA to be expressed in the target cell into which the DNA has been incorporated. The region contained in the DNA comprises a tri-nucleotide sequence. The tri-nucleotide sequence is selected from codons that define at least some amino acid species constituting the protein, and is complementary to at least some codons that are used in the target cell at a frequency of 0.2 or less.

Description

CROSS-REFERENCE TO RELATED PATENT APPLICATION[0001]The present application claims priority under Japanese Patent Application 2008-311106, filed on Dec. 5, 2008, the entire contents of which are hereby incorporated by reference.TECHNICAL FIELD[0002]The present invention relates to a method for suppressing the growth of a target cell by inducing the expression of a protein in the target cell. The present invention further relates to a method for preparing a virus-resistant transformant by introducing DNA into a target cell. Still further, the present invention relates to a vector employed in these methods and a target cell growth-suppressing agent comprising this vector as an active component, and to a virus growth-suppressing agent and a growth-preventing agent.BACKGROUND ART[0003]In principle, the expression of a protein proceeds along the pathway of transcribing the information in DNA coding for the protein into mRNA, and then polymerizing the amino acids specified by the nucleotid...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): C12N15/67C12N15/70
CPCC12N15/67C12N2830/002C12N15/70A61K48/005A61P31/12A61P35/00A61P37/04A61P43/00
InventorKOBAYASHI, HIDEKI
OwnerJAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECHNOLOGY