Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Use of pak4 and crtc1 for treatment or diagnosis of brain degenerative disease

Inactive Publication Date: 2019-01-17
CHUNGBUK NAT UNIV IND ACADEMIC COOPERATION FOUND
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent relates to using a protein called PAK4 and a transcription factor called CRTC1 to treat degenerative brain diseases such as Alzheimer's. The invention is based on the discovery that increased levels of PAK4 and CRTC1 can protect brain cells from damage and improve behavioral symptoms. The patent aims to use PAK4 and CRTC1 activators as therapeutic agents for degenerative brain diseases.

Problems solved by technology

However, the exact cause thereof has not yet been found, and basic researches are being actively conducted to identify the cause.
However, since these drugs are incapable of reviving dead neurons, there are limitations that they are not intended for complete recovery but for control of symptoms.
In addition, side effects of drugs may cause problems with long-term administration.
However, the specific action mechanism and role of PAK4 on a degenerative brain disease have not been elucidated yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of pak4 and crtc1 for treatment or diagnosis of brain degenerative disease
  • Use of pak4 and crtc1 for treatment or diagnosis of brain degenerative disease
  • Use of pak4 and crtc1 for treatment or diagnosis of brain degenerative disease

Examples

Experimental program
Comparison scheme
Effect test

preparation examples

[0088]Materials and Experimental Methods

[0089]The commercial sources of the materials used in the following experiments and the experimental methods are as described below. In addition, the samples from patients with Parkinson's disease used in the experiments were obtained from Victoria Brain Bank Network (VBBN), and the experimental methods were conducted under the conditions approved by the Ethics Review Committee of the Institutional Review Board of Chungbuk National University (approval number: CBNU-IRB-2011-T01).

[0090]Also, the rats used in the experiments were 260 to 280 g of Female Sprague-Dawley rats obtained from Taconi, and kept under 12-hour light / dark cycles while being fed a normal diet. Animal experiments were also conducted under the conditions approved by the Ethics Review Committee of Chungbuk National University for Animal Experiments (approval number: CBNUA-400-12-02).

[0091]In addition, the human tissues used in the experiments were obtained from VBBN, and all ex...

example 1

[0140]Analysis for Neuroprotective Activity of PAK4 in Parkinson's Disease

[0141]Expression levels of pPAK4 and PAK4 in Parkinson's disease were analyzed. As for samples for analysis, the tissue samples obtained from the subjects for experiments in Table 1 above were immunoblotted with pPAK4 and PAK4 antibodies.

[0142]As a result, as shown in FIG. 1, it has been shown that expressions of pPAK4 and PAK4 were all reduced in the samples derived from patients with Parkinson's disease compared to the control samples. In addition, it has been shown that the expression level of TH, a marker of DA neurons, was also reduced in the Parkinson's disease group compared to the control group (see FIGS. 1A and 1B). Besides, the expression levels of pPAK4 and PAK4 in living TH-positive DA neurons were analyzed. As a result, it has been shown that the expression in DA neurons of the Parkinson's disease group was reduced compared to the control groups (age-matched control, young control) (see FIGS. 1C t...

example 2

[0143]Analysis of Expression Levels of PAK4 and Phosphorylated-PAK4 in Animal Model of Parkinson's Disease

[0144]After each brain tissue was obtained through the experimental procedure as described above in the rat animal model of Parkinson's disease induced by administration of 6-OHDA, the expression levels of PAK4 and phosphorylated-PAK4 were analyzed.

[0145]As a result, as shown in FIG. 2, it has been shown that the expression level of phosphorylated-PAK4 was significantly reduced in the samples from rats with Parkinson's disease induced by 6-OHDA, compared to the control without 6-OHDA administration (see FIGS. 2A to 2C).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to use of PAK4 and CRTC1 for the treatment or diagnosis of a degenerative brain disease. Specifically, the present invention relates to a pharmaceutical composition for the prevention or treatment of a degenerative brain disease comprising PAK4 (p21-activated kinase 4) or a PAK4 activator as an effective ingredient. In addition, the present invention relates to a pharmaceutical composition for the prevention or treatment of a degenerative brain disease comprising a CRTC1 expression enhancer or activator as an effective ingredient, a diagnostic kit for a degenerative brain disease comprising an agent for detecting CRTC1, and a method for screening a substance for the prevention or treatment of a degenerative brain disease, comprising (a) applying a candidate drug to brain tissue or brain cells containing CRTC1 gene or CRTC1 protein; (b) measuring the degree of phosphorylation in CRTC1; and (c) determining the candidate drug as a substance for the prevention or treatment of a degenerative brain disease when the measurement in step (b) indicates that the phosphorylation in CRTC1 is increased.

Description

BACKGROUND OF THE INVENTION(a) Field of the Invention[0001]The present invention relates to use of PAK4 and CRTC1 for the treatment or diagnosis of a degenerative brain disease.(b) Description of the Related Art[0002]A degenerative brain disease refers to a disease that occurs in the brain among other degenerative diseases occurring with age, and may be classified according to main symptoms and affected brain areas. It typically includes Alzheimer's disease or Parkinson's disease, or the like. A degenerative brain disease is known to be caused by neuronal death resulting from protein aggregation due to age-related neurodegeneration and other genetic / environmental factors. However, the exact cause thereof has not yet been found, and basic researches are being actively conducted to identify the cause.[0003]Along with Alzheimer's disease, Parkinson's disease is one of the typical degenerative neurological diseases occurring in old age. It affects about 1% of the population aged 65 year...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/50G01N33/68G01N33/53
CPCG01N33/5008G01N33/6812G01N2800/56G01N2800/52G01N2800/2835G01N33/53G01N33/5088G01N33/5058A61K38/16A61K39/395A61K48/005G01N33/5044G01N33/6896G01N2440/14
Inventor KIM, EUNG GOOKWON, SO YOONSHIN, EUN YOUNG
Owner CHUNGBUK NAT UNIV IND ACADEMIC COOPERATION FOUND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com