Medical cornea paster and its preparation method
A patch and cornea technology, applied in the field of medicine, can solve the problems of limited source of materials, limited source of corneal limbus, limited effect, etc.
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[0021] 1. Raw materials:
[0022] (1) Medium a-MEM was purchased from GIBCO BRL (Grand Island, NY, USA), and its components include essential amino acids, proteins and polysaccharides for maintaining cell growth, product number: 1206692.
[0023] (2) Fetal bovine serum (FBS), purchased from Hyclone (Logan, UT), is an essential nutrient for cell growth, and its products are strictly in accordance with the quality standards of fetal bovine serum.
[0024] (3) Trypsin-EDTA, purchased from GIBCO BRL (Grand Island, NY, USA), is used for digesting cells.
[0025] (4) CD34, CD44, CD45, CD38, CD90, CD71, CD147, and HLA-DR were purchased from BD Company and are used to identify antibodies for markers of mesenchymal stem cells.
[0026] (5) Amniotic membrane: taken from the placenta of cesarean section women, preoperative detection of maternal serum HbsAg, anti-HCV, HCV-RNA, anti-HDV, anti-HEV, anti-HIV-1 / 2, HIV-1-RNA, RPR and CMV-DNA are negative, and the test method adopts the clini...
Embodiment 1
[0048] Ocular Surface Epithelial Recovery Evaluation
[0049] Total
[0050] Table 1: Corneal recovery of rats in different control groups 4 weeks after surgery
Embodiment 2
[0052] Restoration of Vision in Burned Rats
[0053] Through the tracking of the visual reflex state of the experimental rats by the optokinetic tracker, the total distance of motion and the trajectory of the single eye before the burn, the single eye before the burn, and the single eye after the burn are compared (see Fig. 6a, Fig. 6b, Fig. 6c, Fig. 6d, Fig. 6e, Fig. 6f), it can be seen that there is a significant difference in the tracking of the visual status of rats by the optomotor tracker, and the optomotor tracker works normally. Different types of cells were transplanted after the burn, and the data of each group were compared horizontally, and the P values were compared through the optokinetic responses of different gratings (see Table 2, Table 3, Table 4, and Table 5, and the comparison between mesenchymal stem cells and other types of cells was obtained). The transplantation method can significantly improve the visual recovery effect of rats.
[0054] Th...
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