Medical cornea paster and its preparation method

Abstract

A medical cornea patch and its preparing method are disclosed, which implants autogenous mesenchyma stem cell to amnion for being carrier to treat cornea disease. The process includes: obtaining bone marrow 10ml from healthy person in clinical practice, diluting by PBS in ratio of 1:1, adding 5ml of Ficoll, centrifuging for 20 minutes, sucking out the nebulous single nuclei cell suspension, washing three times by PBS, implanting to 10% FBS containing culture medium of a-MEM, fetching 2í‡105 cell of the fourth generation to treated amnion with size of 1.5cmí‡1.5cm after 10 to 14 days, adding to culture medium to continue culturing, till the cell grow fully of the whole amnion.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and in particular relates to a patch prepared by planting mesenchymal stem cells on amnion for treating corneal diseases and a preparation method thereof. Background technique: [0002] The treatment of corneal injury is a complicated clinical problem. The cornea is divided into five layers, namely corneal epithelium, anterior boundary layer, corneal stroma layer, posterior boundary layer and corneal endothelial layer. Clinical corneal diseases include physical and chemical burns, mechanical trauma, infection, recurrent pterygium, Steven -Johnson's syndrome, tetanoid, trachoma, etc. Severe conjunctival and corneal diseases are still a major problem for ophthalmologists in the treatment of vision restoration. After corneal burns, a large number of limbal stem cells are lost, the corneal epithelium cannot be repaired normally, the cornea is cloudy, new blood vessels increase and form fibrosis, a...

AI Technical Summary

Problems solved by technology

[0004] 1. Short-term corneal transparency after traditional keratoplasty, if the diagnosis is clear, it cannot be combined with surgery

Its main disadvantage is that only the central φ7mm optically clear area is transplanted, and it is ineffective for the loss of stem cells. Due to the obvious rejection, long-term use of immunosuppressants is required to cooperate with the treatment

[0005] 2. Although there is no immune rejection in autologous corneal limbus transplantation, the source of materials is limited. The main disadvantage is that it increases the risk of disease in healthy eyes. It cannot be transplanted in a 360° circle, and the effect is limited.

[0006] 3. Donor materials for allogeneic limbal transplantation are extremely limited, treatment opportunities are very few, and immune rejection is obvious, long-term medication is required, and the cost is high

Stem cells are the source of cell proliferation and differentiation in self-renewing tissues. Clinically, stem cells solve the problem of organ source caused by organ transplantation. Recently, corneal limbal stem cell transplantation has become a hot spot in the treatment of corneal diseases. The main method is to take autologous or allogeneic Limbal stem cells from the corneal limbus are planted on the amniotic membrane for transplantation to treat corneal diseases. However, due to the limited source of the limbal limbus, the rejection of allogeneic limbal stem cells is obvious after transplantation. Therefore, although it has a significant therapeutic effect, it has not been widely used in clinical practice.

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