Medical cornea paster and its preparation method
A patch and cornea technology, applied in the field of medicine, can solve the problems of less treatment opportunities, increased risk of healthy eyes, limited material sources, etc.
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[0021] 1. Raw materials:
[0022] (1) Medium a-MEM was purchased from GIBCO BRL (Grand Island, NY, USA), and its components include essential amino acids, proteins and polysaccharides for maintaining cell growth, product number: 1206692.
[0023] (2) Fetal bovine serum (FBS), purchased from Hyclone (Logan, UT), is an essential nutrient for cell growth, and its products are strictly in accordance with the quality standards of fetal bovine serum.
[0024] (3) Trypsin-EDTA, purchased from GIBCO BRL (Grand Island, NY, USA), is used for digesting cells.
[0025] (4) CD34, CD44, CD45, CD38, CD90, CD71, CD147, and HLA-DR were purchased from BD Company and are used to identify antibodies for markers of mesenchymal stem cells.
[0026] (5) Amniotic membrane: taken from the placenta of cesarean section women, preoperative detection of maternal serum HBsAg, anti-HCV, HCV-RNA, anti-HDV, anti-HEV, anti-HIV-1 / 2, HIV-1-RNA , RPR, and CMV-DNA are negative, and the test method adopts the cli...
Embodiment 1
[0045] Ocular Surface Epithelial Recovery Evaluation
[0046] Total
[0047] Table 1: Corneal recovery of rats in different control groups 4 weeks after operation
Embodiment 2
[0049] Restoration of Vision in Burned Rats
[0050] The total distance of both eyes moving after operation (0.5cycles / degree)
[0051] Total distance of monocular movement after operation (0.5cycles / degree)
[0052] Average moving speed of both eyes after operation (0.5cycles / degree)
[0053] Average speed of monocular movement after operation (0.5cycles / degree)
[0054] Table 5: Comparison of the average speed of one eye after operation in different groups (P<0.05 has a significant difference)
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