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Method for preparing lycopene oleoresin and lycopene

A technology for lycopene oil and lycopene, which is applied in the field of producing lycopene oleoresin and lycopene, can solve the problems of affecting the quality of lycopene products, easy oxidation of lycopene, large loss of lycopene, etc. Low, no organic solvent residue, less solvent consumption

Inactive Publication Date: 2007-10-10
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current reports are all extracted by organic solvents such as n-hexane, acetone, petroleum ether, chloroform, and ethyl acetate. The solvent consumption is large. During the extraction process, lycopene is easily oxidized by light, air, etc. Large, and the product contains a large amount of organic solvent residues, which greatly affects the quality of lycopene products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Harvest 3L of B. trispora fermentation liquid, filter the fermentation liquid to obtain 900 g of B. trispora mycelium. The mycelium was mixed with 2L of 95% ethanol and stirred for 30 minutes. The treated mycelium was separated by filtration to obtain 540 g of wet mycelium and 2.5 L of ethanol treatment liquid. The wet mycelium was vacuum-dried at 45° C. for 10 hours to obtain 200 g of dried mycelium. The dried mycelium was pulverized with a ball mill at 500 rpm for 1 hour, and then passed through a 300-mesh sieve to obtain a powder sample of B. trispora mycelium.

[0029] Supercritical CO 2 Extraction of B. trispora mycelium

[0030] The 200g B. trispora mycelium powder sample that is obtained is packed into a barrel in a 2L extractor, and sealed. Start supercritical CO 2 The temperature control system of the extractor, the primary separator and the secondary separator, so that the temperatures of the extractor, the primary separator and the secondary separator ar...

Embodiment 2

[0032] Take 40g of the lycopene oleoresin in Example 1 and fully dissolve it in 50ml of ethyl acetate. After standing for stratification, discard the lower layer and filter to obtain 45ml of ethyl acetate solution in which lycopene is dissolved. 50 ml of absolute ethanol was added to the ethyl acetate solution under the protection of nitrogen at 5°C, and the mixture was electromagnetically stirred for 3 hours. The crystalline lycopene was obtained by filtering with a Buchner funnel, the crystals were washed three times with absolute ethanol, and the crystals were collected and vacuum-dried to obtain 2.1 g of lycopene crystals with a purity of 60%.

Embodiment 3

[0034] Take 40g of the lycopene oleoresin in Example 1 and fully dissolve it in 75ml of ethyl acetate. After standing for stratification, discard the lower layer, and filter to obtain 70ml of ethyl acetate solution in which lycopene is dissolved. 210 ml of absolute ethanol was added to the ethyl acetate solution under the protection of nitrogen at 5°C, and the mixture was electromagnetically stirred for 4 hours. Filtrate with a Buchner funnel to obtain crystalline lycopene, wash the crystals with absolute ethanol for 3 times, collect the crystals and carry out vacuum drying. The vacuum drying condition is 40° C. under vacuum, and dry for 2 hours to obtain 1.8 g of lycopene with a purity of 92%. prime crystals.

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PUM

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Abstract

A process for preparing lycopene oil resin and lycopene from a trisporous mold through separating wet mycelia, treating it in alcohol, dewatering, vacuum drying, pulverizing, supercritical CO2 fluid extracting of lycopene to obtain lycopene oil resin, dissolving it in ethyl acetate, and dripping alcohol to obtain crystalline lycopene product.

Description

technical field [0001] The present invention relates to a new method for producing lycopene oleoresin and lycopene, which can be effectively extracted from the fermented mycelia of Mucormyces fungus Blaleslea trispora to obtain lycopene content greater than 60% lycopene crystals and lycopene oleoresin products with more than 3% lycopene. Background technique [0002] Lycopene is a fat-soluble natural pigment belonging to carotenoids. In recent years, studies on the physiological functions of lycopene have found that lycopene has more superior properties than other carotenoids, such as its antioxidant activity is the strongest among carotenoids, and its ability to quench singlet oxygen is V E 100 times that of β-carotene, more than 2 times that of β-carotene, and the ability to scavenge free radicals is also superior to other carotenoids. It has a strong antioxidant effect, can inhibit the growth of cancer cells, slow down the oxidation of low-density lipoprotein (LDL), pre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C12S3/00
Inventor 卢定强胡继军万红贵虞龙欧阳平凯
Owner NANJING TECH UNIV
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