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Bifidobacterium possessing characteristic for anti pathogenesis bacterium in intestinal tract and antioxidation, and application

A technology of Bifidobacterium longum and Escherichia coli, which is applied in the field of microbiology and can solve problems such as potential safety hazards, acid and bile resistance, etc.

Inactive Publication Date: 2008-08-27
王敖喜 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, strain A is less resistant to acid and bile; strain B can effectively inhibit or kill intestinal pathogenic bacteria; strain C has excellent adhesion to intestinal cells; strain D can inhibit tumors; There are certain hidden dangers in sex, etc.
[0004] However, there is no bifidobacterium that not only has safety, but also has multiple advantages such as acid resistance, bile resistance, and bacteriostasis.

Method used

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  • Bifidobacterium possessing characteristic for anti pathogenesis bacterium in intestinal tract and antioxidation, and application
  • Bifidobacterium possessing characteristic for anti pathogenesis bacterium in intestinal tract and antioxidation, and application
  • Bifidobacterium possessing characteristic for anti pathogenesis bacterium in intestinal tract and antioxidation, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Bifidobacterium longum BM358 was isolated from healthy and long-lived elderly in five longevity towns in the world

[0050] Bama Yao Autonomous County in Guangxi is one of the areas with a relatively high concentration of long-lived elderly people in my country. In 1991, it was rated as the fifth longest-lived town in the world by the International Association of Natural Medicine. Take antibiotics. In 1999, the present inventor selected 15 healthy and long-lived old people over 90 years old from Bama Yao Autonomous County, Guangxi, collected the middle and back sections of their feces, and kept them in cold storage (about 0° C.) until the test.

[0051] Then, mash the feces with a sterile glass rod, and after a while, take out 1mL of the supernatant, put it into 9mL of the diluent, and dilute to 10% in turn according to the ten-fold dilution method. -7 , 10 -8 , 10 -9 . Take 1mL of the bacterial solution with different dilution factors in a sterile petri dish, pour i...

Embodiment 2

[0061] Safety of Bifidobacterium longum strain BM358 administered by continuous gavage to Kunming mice

[0062] 80 Kunming mice, half male and half male, were randomly divided into 4 groups. Bifidobacterium BM358 was inoculated into MRS medium, and placed in anaerobic culture at 36±1°C for 48 hours. The stock solution and 5-fold concentrated solution of the culture were respectively , orally with 20mL / KgBW to the mice for continuous gavage for 3 days, and observed for 7 days.

[0063] The results are shown in Table 1 below. Compared with the control group, no toxic reaction or death was observed in the tested mice.

[0064] Table 1 Bifidobacterium longum BM358 live bacterium is to mouse toxic effect death list

[0065]

Embodiment 3

[0067] Safety of intraperitoneal injection of Bifidobacterium longum strain BM358 to healthy adult ICR mice

[0068] 40 healthy adult ICR mice, half male and half female, the weight range of female mice is 19.5-22.0g, and the weight range of male mice is 20.0-22.2g. Divide into 4 groups at random, transfer Bifidobacterium longum BM358 strains to MRS solid medium for culture, after anaerobic culture at 36±1°C for 48 hours, scrape the bacterial lawn on the plate, and make bacterial suspension with MRS liquid medium , adjust the density to 5×10 9 cfu / mL, the mice were intraperitoneally injected with 0.3mL of bacterial suspension every day, and the reaction was continuously observed for 7 days.

[0069] The results are shown in Table 2 below, no toxic reaction or death was found in the recipient mice.

[0070] Table 2 Bifidobacterium longum BM358 is to mouse toxicity result

[0071]

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Abstract

This invention describes a bifidobacterium, i.e., bifidobacterium longum BM358 that can resist intestinal pathogens as well as resist oxidation. The bifidobacterium longum has such properties as high security, outstanding acid and bile resistance, adhesion to intestinal epithelial cells, outstanding intestinal pathogen resistance and oxidation resistance. The bifidobacterium longum can be used to prevent and treat gastritis, gastrelcosis and duodenitis caused by pylorus helicobacteria, and enteritis, acute diarrhea and enterogastric disorder caused by colibacillus and other intestinal pathogens.

Description

technical field [0001] The present invention relates to microbiology. More specifically, it relates to a bifidobacterium with anti-intestinal pathogenic bacteria and anti-oxidation properties and a food composition or a pharmaceutical composition containing the live bacterial strain. Background technique [0002] Bifidobacterium is one of the most important physiological bacteria in the human intestinal tract. It adheres to the intestinal epithelial cells and forms a stable flora, which is very important for maintaining the host intestinal micro-ecological balance, enhancing immune function, anti-tumor, anti-infection, Anti-aging and other aspects have played an important role. However, the vast majority of bifidobacteria are strictly anaerobic, have relatively strict nutritional requirements, and have poor gastric acid and bile properties. [0003] It is worth noting that even within the same genus and species of Bifidobacteria, there are obvious differences in their prop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/74A61P1/00A61P1/04A61P31/04C12R1/01A61K35/745
Inventor 王敖喜任大宝
Owner 王敖喜
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