Sulfate-reducing bacteria testing fluid and testing bottle

A technology of sulfate and test liquid, applied in the field of test liquid of sulfate-reducing bacteria and test bottles thereof, can solve the problems of error in test results, cumbersome and complicated operation procedures, etc., to avoid pollution and interference, to be convenient to use, and to save time. long effect

Inactive Publication Date: 2009-06-10
白莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operating procedures are cumbersome, complex and lengthy, and carelessness in any link may cause errors in test results

Method used

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  • Sulfate-reducing bacteria testing fluid and testing bottle

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Effect test

Embodiment 1

[0034] Sulfate-reducing bacteria test solution formula: sodium lactate 2‰, sodium chloride 10‰, magnesium sulfate 1‰, yeast extract 3‰, ferrous sulfate 0.5‰, vitamin C 0.1‰, sodium acetate 5‰ and distilled water 978.4‰ ‰composition.

[0035] After preparing the above-mentioned test solution, adjust the pH value to 6.5-7.2, pack it in sterile, colorless and transparent small glass bottles with 4.5 ml per bottle, and then sterilize it by high pressure.

[0036] Sulfate Reducing Bacteria Test Bottle Implementation Method: See figure 1 , in the figure 1 is an aluminum skin cover, 2 is a rubber stopper, 3 is a glass bottle with equal cross-section, and 4 is a test solution; H 1 is the height of the test liquid, H 2 For the height of the void, the test bottle is 4.5 milliliters in the present embodiment, and the height H of the test solution is 1 is 3 cm, the clearance height H 2 is 1.5 cm.

Embodiment 2

[0038] Sulfate-reducing bacteria test solution formula is: calcium lactate 10‰, potassium chloride 3‰, magnesium sulfate 5‰, yeast extract 1‰, ferrous ammonium sulfate 0.1‰, vitamin C0.5‰, acetic acid 10‰ and distilled water 970.4‰ composition.

[0039] After preparing the above-mentioned test solution, adjust the pH value to 6.5-7.2, pack it in sterile, colorless and transparent small glass bottles with 4.5 ml per bottle, and then sterilize it by high pressure.

[0040] Sulfate Reducing Bacteria Test Bottle Implementation Method: See figure 1 , in the figure 1 is an aluminum skin cover, 2 is a rubber stopper, 3 is a glass bottle with equal cross-section, and 4 is a test solution; H 1 is the height of the test liquid, H 2 For the height of the void, the test bottle is 4.5 milliliters in the present embodiment, and the height H of the test solution is 1 is 4 cm, the clearance height H 2 is 1.2 cm.

Embodiment 3

[0042] Sulfate-reducing bacteria test solution formula is: sodium lactate 6‰, potassium chloride 7‰, magnesium sulfate 3‰, yeast extract 2‰, ferrous ammonium sulfate 0.3‰, vitamin C0.3‰, acetic acid 8‰ and distilled water 973.4‰ ‰composition.

[0043] After preparing the above-mentioned test solution, adjust the pH value to 6.5-7.2, pack it in a sterile, colorless transparent small glass bottle with 9.0 ml per bottle, and then sterilize it by high pressure.

[0044] Sulfate-reducing bacteria test bottle implementation method: see figure 1 , in the figure 1 is an aluminum skin cover, 2 is a rubber stopper, 3 is a glass bottle with equal cross-section, and 4 is a test solution; H 1is the height of the test liquid, H 2 For the height of the void, the test bottle is 9.0 milliliters in the present embodiment, and the height H of the test solution is 1 is 3.5 cm, the clearance height H 2 is 2.8 cm.

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Abstract

A sulfate reducing bacterium test liquid and its test tube are disclosed. The sulfate reducing bacterium test liquid consists of lactate 2-10wt per mill, chloride 3-10wt per mill, sulfate 1-10 per mill,yeast extract 1-3 per mill,perferrite 0.1-1.0 per mill, vitamins 0.1-0.5 per mill, acetic acid or acetate 1.0-10 per mill and distilled water 955.5-991.8 per mill. Height H1 of test liquid: space height H2=1:0.3-0.8. It's fast, convenient, correct and has long storage time.

Description

[0001] One, technical field: the present invention relates to a kind of test method of bacterium and test apparatus thereof, particularly relate to a kind of test liquid and test bottle thereof of sulfate-reducing bacteria in other materials such as water quality, sludge, sediment. 2. Background technology: [0002] Sulfate-reducing bacteria are a kind of organic chemical heterotrophic bacteria, widely distributed in nature. Under anaerobic conditions, sulfate-reducing bacteria are more present in water supply, drainage pipes, sludge, and sediments, and they can use organic matter attached to metal surfaces as a carbon source and utilize the organic matter produced in bacterial biofilms. Hydrogen, reducing sulfate to hydrogen sulfide. Hydrogen sulfide is extremely corrosive and can chemically react with metal substrates to cause chemical corrosion. The iron sulfide formed by the reaction is deposited on the metal surface, changing the surface properties of the base metal. At...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12M1/34
Inventor 刘五一白莉崔纪周李树华张玉忠
Owner 白莉
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