Enterobacteria containing tablet
A technology for bacteria and tablets, applied in the field of tablets containing intestinal bacteria, can solve the problems of easy inactivation and decreased survival rate of viable bacteria.
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Embodiment 1
[0039] [Example 1] Production of Tablets Containing Enteric Bacteria
[0040] [cultivation of bacteria]
[0041] A culture solution of Bifidobacterium bifidum enriched in advance with a GAM medium (manufactured by Nissui Pharmaceutical Co., Ltd.) was inoculated in the same medium at 0.1% by weight, and cultured at 37° C. for 16 hours. It was centrifuged, and the obtained precipitate was regarded as wet bacterial cells.
[0042] [Preparation of bacterial suspension]
[0043] A dispersion medium prepared by adding dextrin to an aqueous solution prepared at a concentration of 0.5M mannitol, sodium glutamate 0.5M, arginine 0.1M, sodium succinate 0.1M, and magnesium sulfate 0.01M , the obtained wet cells were suspended.
[0044] [Manufacture of fungus powder by spray drying]
[0045] The bacterial suspension prepared as above was spray-dried using a spray dryer equipped with a four-channel nozzle (as described in Japanese Patent No. 2797080). That is to say, the bacterial susp...
Embodiment 2
[0048] [Example 2] The mensuration of the number of live bacteria in tablet raw material and tablet
[0049] The number of live bacteria in the tableting raw materials and tablets was measured by the following method. For tablets, lightly crush to powder and mix well. Precisely measure 10.0 g of each of the tableting raw material and the powdered tablet, and add the diluent to make the total amount 100 mL. Then shake and mix vigorously, and then shake and mix again after incubating at 35-37°C for about 30 minutes. Accurately measure 1mL, repeat the operation of adding to another 9mL of diluent that has been accurately separated, and use the solution containing about 20 to 200 viable bacteria in 1mL as the test solution. 1 mL of the test solution was added to each of three petri dishes, and 20 mL of the agar medium for the quantification of the two-tailed bacteria maintained at 45-48° C. was added to each of the three petri dishes, and mixed rapidly and solidified. Under the...
Embodiment 3
[0080] [Example 3] Measurement of Survival Rate during Tablet Production
[0081] The survival rate at the time of production was calculated by the following formula.
[0082] Survival rate (%) at the time of tablet making = the number of viable bacteria in 1g tablet / the number of viable bacteria in 1g tablet raw material × 100
[0083] In addition, the hardness of a tablet was measured with the tablet breaking strength tester (TH-203RP manufactured by Toyama Sangyo Co., Ltd.).
[0084] Table 1
[0085] the first time the second time the third time average Average hardness of tablet (N) 53.9 55.86 62.72 57.526 The number of viable bacteria in the tablet raw material
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