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Method for improving single batch conversion bubatrate concentration in alloisomerism preparation of styrene glycol

A phenylethylene glycol, stereoisomeric technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as low substrate concentration

Inactive Publication Date: 2009-07-15
JIANGNAN UNIV
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  • Description
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  • Application Information

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Problems solved by technology

[0007] At present, our laboratory has screened out the strain Candida parapsilosis CCTCC M203011 which can stereoselectively transform and prepare optically pure phenylglycol, but the substrate concentration of the transformation is too low, and 10% of the cells Concentration, single batch conversion substrate concentration up to 1.5%

Method used

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  • Method for improving single batch conversion bubatrate concentration in alloisomerism preparation of styrene glycol

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Effect test

Embodiment 1

[0036] In 5ml, 0.1mol / L potassium phosphate buffer solution (pH6.5), add 0.5g CCTCC M203011 bacterial cells and 0.2g NKA II resin and 0.10g racemic phenylethylene glycol (substrate concentration 2% ), shaking and reacting on a constant temperature shaker at 30° C. for 48 hours. After the reaction, the mixture was centrifuged, and 0.5 ml of the supernatant was extracted with 3 ml of ethyl acetate. The optical purity of the product (S)-PED was 94.31% e.e., and the yield was 83.63%.

Embodiment 2

[0038] In 5ml, 0.1mol / L potassium phosphate buffer solution (pH6.5), add 0.5g CCTCC M203011 bacterial cells and 0.25g NKA II resin and 0.125g racemic phenylethylene glycol (substrate concentration 2.5%) ), shaking and reacting on a constant temperature shaker at 30° C. for 72 hours. After the reaction, the mixture was centrifuged, and 0.5 ml of the supernatant was extracted with 3 ml of ethyl acetate. The optical purity of the product (S)-PED was 96.64% e.e., and the yield was 89.67%.

Embodiment 3

[0040] In 5ml, 0.1mol / L potassium phosphate buffer solution (pH6.5), add 0.5g CCTCC M203011 bacterial cells and 0.30g NKA II resin and 0.15g racemic phenylethylene glycol (substrate concentration 3.0%) ), shaking and reacting on a constant temperature shaker at 30°C for 96 hours, after the reaction, the mixture was centrifuged, and 0.5ml of the supernatant was extracted with 3ml of ethyl acetate. The optical purity of the product (S)-PED was 98.33%e.e., and the yield was 83.57%.

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Abstract

The invention discloses a method for preparing (S) phenylethylene glycol through stereoisomerism and increasing the concentration of a single batch of conversion substrate, which belongs to the technical field of biological separation of racemic compounds. The present invention prepares (S) phenylglycol reaction system in stereoisomerism, and system pH is 6.5, uses Candida parapsilosis (Candida parapsilosis) CCTCCM203011 as transformation strain, adds medium polar macroporous resin NKA II, improves Transform substrate concentrations up to 2-fold in a single batch.

Description

technical field [0001] The invention discloses a method for preparing (S) phenylethylene glycol through stereoisomerism and increasing the concentration of a single batch of conversion substrate, which belongs to the technical field of biological separation of racemic compounds. Background technique [0002] Optically pure phenylethylene glycol, that is, (S)-phenylethylene glycol, also known as (S)-PED, is not only an indispensable and important chiral additive in liquid crystal materials, but also has become a As an important intermediate of medicine, pesticide and functional materials, it is very meaningful to carry out research on the resolution method of phenylethylene glycol. [0003] Chiral compounds play an important role in people's lives. Since the two enantiomers are different in pharmacology, toxicology and functional effects, the preparation of optically pure chiral modular compounds is very important in medicine, agriculture, materials and Environmental protect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/02C12P41/00C12R1/72
Inventor 徐岩刘鑫勤穆晓清
Owner JIANGNAN UNIV
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