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Method for extracting wheat bran protein through two-step enzymolysis

A wheat bran and enzymatic hydrolysis technology is applied in the field of bioengineering, which can solve the problems of unsuitable large-scale production, high extraction process cost, and many protein impurities, and achieves the advantages of saving consumption, reducing energy consumption and reducing substrate concentration. Effect

Inactive Publication Date: 2017-12-08
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

Alkaline extraction is simple to operate, easier to control the process conditions, and the extraction efficiency is relatively high, so it is suitable for large-scale industrial production. Low; enzymatic extraction mainly uses protease, because protease can modify and degrade some plant proteins to obtain some small molecule soluble proteins and soluble peptides, and the reaction conditions of enzyme extraction are mild and the by-products are less. It is a commonly used method today. The method of producing high-quality protein; the extraction master uses ethanol to extract the protein in the bran. The protein extracted by this method contains more impurities and the extraction process cost is relatively high, so it is not suitable for large-scale production

Method used

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  • Method for extracting wheat bran protein through two-step enzymolysis
  • Method for extracting wheat bran protein through two-step enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Take the wheat bran residue after removing the starch, add deionized water to adjust the mash concentration to 15%, adjust the pH of the slurry to 5.5 and the temperature to 50°C with hydrochloric acid with a mass fraction of 5%, based on the weight of the wheat bran residue The total mass was added to the cell wall-breaking enzyme, and the added amount was 0.2%, and the pretreated wheat bran slurry was obtained after reacting for 2 hours.

[0021] (2) adding distilled water to the slurry obtained in step (1) in step (1) to adjust the slurry again until the mash concentration is 10%, and adjusting the pH to 8.0 with a mass concentration of 4% sodium hydroxide solution, setting the temperature At 80°C, based on the total weight of wheat bran, Novozymes’ Alcalase 2.4L FG was added in an amount of 2.0%, centrifuged after reaction 2, and the centrifugal speed was 4000rpm. The supernatant is concentrated and freeze-dried to obtain the gluten protein product.

Embodiment 2

[0023] Except that the mash concentration in step (1) was set to 10%. Gluten was extracted in the same manner as in Example 1. The results are shown in Table 1, the protein extraction rate of step (1) was 3%.

Embodiment 3

[0025] Except that the mash concentration in step (1) is set to 20%. Gluten was extracted in the same manner as in Example 1. Result is as shown in table 1, and the protein extraction rate of step (1) is 4.7%

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Abstract

The invention discloses a method for extracting wheat bran protein through two-step enzymolysis, and relates to the field of biological engineering. The method comprises the following steps: (1) taking wheat bran residues, adding distilled water for pulping, adjusting pH value and temperature, adding lyticase for reaction to obtain pretreated pulp; (2) directly adding the obtained pulp into the distilled water for pulping again, adjusting the pH value and the temperature without other treatments, adding alkaline protease to react for 1-6 hours, separating centrifugally, taking supernatant to be freeze-dried to obtain a protein product. Compared with other enzymatic extraction methods, the method can fully increase the extraction rate of rice bran protein, and is especially suitable for wheat brans treated at a high temperature.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for extracting wheat gluten protein, in particular to a method for extracting wheat gluten protein by two-step enzymatic hydrolysis. Background technique [0002] As a by-product of the milling industry, wheat bran is abundant and cheap. From the perspective of plant histology, wheat bran consists of pericarp, seed coat, nacelle and aleurone layer in sequence. The wheat bran layer cannot be completely separated during the flour production process, so in addition to the cortex containing the wheat grain, the wheat bran also contains the germ and endosperm layers. Therefore, in general, wheat bran contains 12% water, 13% protein, 4% fat, 45% dietary fiber and some other carbohydrates. [0003] The protein in wheat bran contains a higher proportion of lysine, arginine, alanine, aspartic acid and glycine and less glutamine, proline, phenylalanine than wheat protein amino aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 王建勋侯同刚咸漠王乔董晴晴
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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